Telomeric Repeat Binding Factor 2 (TERF2) antibody

Details for Product No. ABIN189464
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Antigen
Synonyms TERF2, TRBF2, TRF2, trf2
Reactivity
Hamster, Human, Monkey, Mouse (Murine), Rat (Rattus)
(42), (10), (6), (3), (3), (2), (1)
Host
Rabbit
(26), (11), (9)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(41), (16), (12), (10), (9), (8), (8), (4), (3), (3), (2), (2), (1), (1)
Pubmed 4 references available
Quantity 0.1 mL
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Catalog No. ABIN189464
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Immunogen Baculovirus purified TRF2 protein.
Purification affinity purified
Alternative Name TERF2
Background TRF2 is a ubiquitously expressed protein that is implicated in the control of telomerelength. TRF2, like TRF1, contains a Myb-related DNA binding motif. It binds to duplexTTAGGG repeats and is localized to all human telomeres in metaphase chromosomes.TRF2 is thought to protect chromosome ends by maintaining the correct structure attelomere termini. The use of mutant forms of TRF2 has implied a role for TRF2 in theprevention of senescence in primary human cells. Recently, it has been shown thatinhibition of TRF2 resulted in apoptosis in a subset of mammalian cell types. TRF2 isoverexpressed in a number of human tumors and overexpression of TRF2 may beoncogenic. Alternate Names: anti-Telomeric DNA binding protein antibody, anti-Telomeric repeat binding factor 2antibody, anti-Telomeric repeat binding protein 2 antibody, anti-TERF 2 antibody,anti-TERF2 antibody, anti-TRBF 2 antibody, anti-TRBF2 antibody, anti-TRF 2 antibody,anti-TTAGGG repeat binding factor 2 antibody.
Gene Symbol: TERF2
Gene ID 7014
Research Area DNA/RNA, Cell Cycle, Enzymes
Application Notes This TRF2 antibody is useful for ChIP, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot, where a band at ~56 kDa is seen. Immunoprecipitation was reported in scientific literature. In ICC/IF, nuclear staining was observed in HeLa cells. In IHC, nuclear staining was observed in xenografted human breast cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Recommended dilutions: Chromatin Immunoprecipitation 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry 1:200, Immunohistochemistry-Paraffin 1:200, Immunoprecipitation, Western Blot 1:2000-1:5000
Protocol Western Blot Protocol Specific for NB110-57130
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.Immunocytochemistry Protocol Specific for NB110-57130 Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.
. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-glycine, 150 mM NaCl, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
anti-Telomeric Repeat Binding Factor 2 (TERF2) antibody Detection of TRF2 in HeLa nuclear extract using ABIN189464.
General Smogorzewska, van Steensel, Bianchi et al.: "Control of human telomere length by TRF1 and TRF2." in: Molecular and cellular biology, Vol. 20, Issue 5, pp. 1659-68, 2000 (PubMed).

Zhu, Kuester, Mann et al.: "Cell-cycle-regulated association of RAD50/MRE11/NBS1 with TRF2 and human telomeres." in: Nature genetics, Vol. 25, Issue 3, pp. 347-52, 2000 (PubMed).

Smith, de Lange: "Tankyrase promotes telomere elongation in human cells." in: Current biology : CB, Vol. 10, Issue 20, pp. 1299-302, 2001 (PubMed).

Smogorzewska, de Lange: "Different telomere damage signaling pathways in human and mouse cells." in: The EMBO journal, Vol. 21, Issue 16, pp. 4338-48, 2002 (PubMed).

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