Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (C-Term) antibody

Details for Product No. ABIN189476
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Antigen
Epitope
C-Term
(38), (7), (6), (5), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(201), (30), (28), (2), (1), (1)
Host
Rabbit
(125), (74), (6), (2), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(98), (69), (65), (57), (34), (30), (16), (16), (10), (9), (2), (1), (1)
Pubmed 4 references available
Quantity 0.1 mL
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Catalog No. ABIN189476
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Immunogen A synthetic peptide from C-terminus of human Ki-67.
Clone SP6
Isotype IgG
Specificity This antibody only detects AMPK alpha 1 phosphorylated at Threonine 183 and AMPK alpha 2 phosphorylated Threonine 172.
Purification Tissue culture supernatant rabbit ascites
Alternative Name Ki-67
Background Ki-67 antigen is a nuclear antigen specifically associated with cell proliferation. Ki-67 isexpressed in all proliferating cells which are in the active phases of the cell cycle (lateG1, S, G2, and mitosis), but absent in resting cells. In G1, it is predominately localized inthe perinucleolar region and also found in nuclear matrix in the later phases (1). Ki-67 isthought to be involved in the maintaining of cell proliferation, however, its exactmechanism for function is unknown. Due to the fact that Ki-67 can be used to determinethe growth fraction (the proportion of the cells committed to the cell cycle), it is consideran important marker in human tumor diagnostics and prognostics (2). Alternate Names: anti-Antigen identified by monoclonal antibody, anti-Ki 67 antibody, anti-Antigen KI67antibody, anti-KIA antibody, anti-MKI67 antibody, anti-Proliferation related Ki 67 antigenantibody.
Gene Symbol: MKI67
Gene ID 4288, 291234
Research Area Proliferation Markers, Cell Cycle
Application Notes This product is useful for: Western Blot, Immunoprecipitation. In Western blot this antibody detects a band at approximately 62/63kDa.
Recommended dilutions: Immunohistochemistry, Immunohistochemistry-Paraffin 1:200
Protocol Protocol specific for Ki67 Antibody < p class="MsoNormal"> 

< p class="MsoNormal">< span class="textboldblack">Immunohistochemistry Protocol for Paraffin-embedded Tissues

< span class="textboldblack">1. < span class="textboldblack">Solutions and reagents

< span class="textboldblack">1.1. Xylene

< span class="textboldblack">1.2. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)

< span class="textboldblack">1.3. Washing buffer:
TBST washing buffer: 1XTBS/0.1 % Tween-20
To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.
Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.

< span class="textboldblack">1.4. Distilled water (dH2O)

< span class="textboldblack">1.5. Antigen Retrieval Solution:
0.01M Sodium Citrate
Restrictions For Research Use only
Format Liquid
Buffer Tissue culture supernatant with 0.05 % BSA, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (C-Term) antibody Immunohistochemical staining of paraffin-embedded Human tonsil stained with anti-Ki-67 RabMAb (Cat. #110-57147)
General Gerdes: "Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies." in: Seminars in cancer biology, Vol. 1, Issue 3, pp. 199-206, 1991 (PubMed).

Scholzen, Gerdes: "The Ki-67 protein: from the known and the unknown." in: Journal of cellular physiology, Vol. 182, Issue 3, pp. 311-22, 2000 (PubMed).

Popa, Zubkova, Medvedovic et al.: "Regeneration of the adult thymus is preceded by the expansion of K5+K8+ epithelial cell progenitors and by increased expression of Trp63, cMyc and Tcf3 transcription factors in the thymic stroma." in: International immunology, Vol. 19, Issue 11, pp. 1249-60, 2007 (PubMed).

Klinakis, Szabolcs, Chen et al.: "Igf1r as a therapeutic target in a mouse model of basal-like breast cancer." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 7, pp. 2359-64, 2009 (PubMed).

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