Antigen Identified By Monoclonal antibody Ki-67 (MKI67) antibody

Antigen: Antigen Identified By Monoclonal Antibody Ki-67 (MKI67)

Synonyms: KIA, Ki67, Ki-67, D630048A14Rik, MKI67, kia, mki67, MGC132156

Clonality: Monoclonal (SP6)

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN189476

Additional Information

Alternative name: Ki-67

Gene ID: 4288, 291234

UniProt: P46013

Immunogen: A synthetic peptide from C terminus of human Ki67.

Cross-Reactivity: Human

Format: Supernatant

Isotype: IgG

Clone: SP6

Description: Ki-67 antigen is a nuclear antigen specifically associated with cell proliferation. Ki-67 isexpressed in all proliferating cells which are in the active phases of the cell cycle (lateG1, S, G2, and mitosis), but absent in resting cells. In G1, it is predominately localized inthe perinucleolar region and also found in nuclear matrix in the later phases (1). Ki-67 isthought to be involved in the maintaining of cell proliferation, however, its exactmechanism for function is unknown. Due to the fact that Ki-67 can be used to determinethe growth fraction (the proportion of the cells committed to the cell cycle), it is consideran important marker in human tumor diagnostics and prognostics (2). Alternate Names: anti-Antigen identified by monoclonal antibody, anti-Ki 67 antibody, anti-Antigen KI67antibody, anti-KIA antibody, anti-MKI67 antibody, anti-Proliferation related Ki 67 antigenantibody.

Specificity: A synthetic peptide from C-terminus of human Ki-67. Species Reactivity: Reacts in human, mouse, and rat. Not tested in other species.

Application Details

Application Notes: Suggested working dilutions: immunohistochemistry 1/200

Purity: Tissue culture supernatant rabbit ascites

Purification: Supernatant

Buffer: 0.05% BSA. Preservative: 0.1% sodium azide.

Storage: Store at 4C. Do not freeze.

Research Area: Proliferation Markers, Cell Cycle

Restrictions: For Research Use only

Images

Immunohistochemical staining of paraffin-embedded Human tonsil stained with anti-Ki-67 RabMAb (Cat. #110-57147)

Publications

Gerdes: "Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies." in: Seminars in cancer biology, Vol. 1, Issue 3, pp. 199-206, 1991 (PubMed).

Scholzen, Gerdes: "The Ki-67 protein: from the known and the unknown." in: Journal of cellular physiology, Vol. 182, Issue 3, pp. 311-22, 2000 (PubMed).

Popa, Zubkova, Medvedovic et al.: "Regeneration of the adult thymus is preceded by the expansion of K5+K8+ epithelial cell progenitors and by increased expression of Trp63, cMyc and Tcf3 transcription factors in the thymic stroma." in: International immunology, Vol. 19, Issue 11, pp. 1249-60, 2007 (PubMed).

Klinakis, Szabolcs, Chen et al.: "Igf1r as a therapeutic target in a mouse model of basal-like breast cancer." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 7, pp. 2359-64, 2009 (PubMed).