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Melan A (MLANA) (N-Term) antibody

Details for Product No. ABIN189510, Supplier: Login to see
Antigen
  • A930034P04Rik
  • Mart1
  • MART-1
  • MART1
Epitope
N-Term
20
17
16
13
12
12
4
3
2
2
1
1
1
1
Reactivity
Human
199
29
24
11
2
1
Host
Rabbit
124
82
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
6
5
4
3
3
3
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
134
73
56
56
49
35
31
20
11
4
2
2
2
1
Supplier
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Immunogen A synthetic peptide corresponding to residues on the N-terminus of human MART-1 was used as an immunogen.
Clone EP1422Y
Isotype IgG
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Tissue culture supernatant
Alternative Name MART-1 (MLANA Antibody Abstract)
Background MART-1 has been widely studied as a melanoma-specific antigen and as amelanosome-specific marker. A role for MART-1 in pigmentation is expected becauseits expression pattern and subcellular distribution is quite similar to the othermelanosomal proteins and usually correlates with melanin content. MART-1 forms acomplex with Pmel17 and affects its expression, stability, trafficking, and the processingwhich is required for melanosome structure and maturation. MART-1 is indispensablefor Pmel17 function and thus plays an important role in regulating mammalianpigmentation (1). A gene has been identified that encodes a melanocyte lineage-specificprotein (MART-1, melanoma antigen recognized by T cells 1) that is a widely sharedmelanoma antigen recognized by the T lymphocytes of patients with establishedmalignancy. Identification of this gene opens possibilities for the development ofimmunotherapies for patients with melanoma (2). Alternate Names: anti-Antigen LB39 AA antibody, anti-Antigen SK29 AA antibody, anti-MART 1 antibody,anti-MART1 antibody, anti-Melan A antibody, anti-Melan A protein antibody,anti-Melanoma antigen recognized by T cells 1 antibody, anti-MLAN A antibody,anti-MLANA antibody.
Gene Symbol: MLANA
Gene ID 2315, 77836, 293890
Research Area Cancer
Pathways
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin. In Western blot this antibody detects a band at approximately 20kDa.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:100-250, Immunoprecipitation (Negative), Western Blot 1:1000-10000
Protocol Protocol specific for Melan-A Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
Western Blotting (WB) image for anti-Melan A (MLANA) (N-Term) antibody (ABIN189510) Western blot analysis on melanoma lysate using anti-MART-1 RabMAb (cat. # 110-57185),...
Immunohistochemistry (IHC) image for anti-Melan A (MLANA) (N-Term) antibody (ABIN189510) Immunohistochemical staining of paraffin-embedded melanoma using anti-MART-1 RabMAb (...
Product cited in: Massoumi, Kuphal, Hellerbrand et al.: "Down-regulation of CYLD expression by Snail promotes tumor progression in malignant melanoma." in: The Journal of experimental medicine, Vol. 206, Issue 1, pp. 221-32, 2009 (PubMed).

Hoashi, Watabe, Muller et al.: "MART-1 is required for the function of the melanosomal matrix protein PMEL17/GP100 and the maturation of melanosomes." in: The Journal of biological chemistry, Vol. 280, Issue 14, pp. 14006-16, 2005 (PubMed).

Background publications Kawakami, Eliyahu, Delgado et al.: "Cloning of the gene coding for a shared human melanoma antigen recognized by autologous T cells infiltrating into tumor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 9, pp. 3515-9, 1994 (PubMed).