Melan A (MLANA) antibody
| Antigen |
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| Synonyms |
MART1, MART-1 |
| Clonality |
Monoclonal (EP1422Y) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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| Catalog no. |
ABIN189510 |
| Quantity |
0.1 ml |
| Price |
Product not available in this region.
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| Shipping to |
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Alternative name
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MART-1
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Gene ID
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2315, 77836, 293890
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UniProt
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Q16655
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Cross-Reactivity
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Human
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Isotype
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IgG
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Clone
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EP1422Y
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Description
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MART-1 has been widely studied as a melanoma-specific antigen and as amelanosome-specific marker. A role for MART-1 in pigmentation is expected becauseits expression pattern and subcellular distribution is quite similar to the othermelanosomal proteins and usually correlates with melanin content. MART-1 forms acomplex with Pmel17 and affects its expression, stability, trafficking, and the processingwhich is required for melanosome structure and maturation. MART-1 is indispensablefor Pmel17 function and thus plays an important role in regulating mammalianpigmentation (1). A gene has been identified that encodes a melanocyte lineage-specificprotein (MART-1, melanoma antigen recognized by T cells 1) that is a widely sharedmelanoma antigen recognized by the T lymphocytes of patients with establishedmalignancy. Identification of this gene opens possibilities for the development ofimmunotherapies for patients with melanoma (2). Alternate Names: anti-Antigen LB39 AA antibody, anti-Antigen SK29 AA antibody, anti-MART 1 antibody,anti-MART1 antibody, anti-Melan A antibody, anti-Melan A protein antibody,anti-Melanoma antigen recognized by T cells 1 antibody, anti-MLAN A antibody,anti-MLANA antibody.
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Specificity
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A synthetic peptide corresponding to residues on the N-terminus of human MART-1 wasused as an immungen. Species Reactivity: Cross reacts in Human. Does not react in Mouse or Rat.
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Application Notes
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Suggested working dilutions: immunohistochemistry 1:100-250,Western Blot 1:1000
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Purification
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Aff - Purified
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Buffer
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50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. Preservative: 0.01% sodium azide.
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Storage
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Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Research Area
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Cancer
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Restrictions
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For Research Use only
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Kawakami, Eliyahu, Delgado et al.: "Cloning of the gene coding for a shared human melanoma antigen recognized by autologous T cells infiltrating into tumor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 9, pp. 3515-9, 1994 (PubMed).
Hoashi, Watabe, Muller et al.: "MART-1 is required for the function of the melanosomal matrix protein PMEL17/GP100 and the maturation of melanosomes." in: The Journal of biological chemistry, Vol. 280, Issue 14, pp. 14006-16, 2005 (PubMed).
Massoumi, Kuphal, Hellerbrand et al.: "Down-regulation of CYLD expression by Snail promotes tumor progression in malignant melanoma." in: The Journal of experimental medicine, Vol. 206, Issue 1, pp. 221-32, 2009 (PubMed).
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Alternatives for antigen "Melan A (MLANA)", type "Antibodies"
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Hosts
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Rabbit (15), Mouse (13)
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Reactivities
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Human (23), Horse (Equine) (1)
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Applications
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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (20), Western Blotting (WB) (10), Immunohistochemistry (IHC) (5), ELISA (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Immunocytochemistry (ICC) (3), Immunofluorescence (IF) (3), Flow Cytometry (FACS) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2)
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Epitopes
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Center (1), N-Term (1)
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2013 BioMed Central Research Awards
