PI3 Kinase P85 alpha (C-Term) antibody

Details for Product No. ABIN189672
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Antigen
Synonyms AA414921, C530050K14, PI3K, p50alpha, p55alpha, p85alpha, AGM7, GRB1, p85, p85-ALPHA, PI3KA
Epitope
C-Term
(30), (21), (15), (13), (10), (10), (9), (7), (6), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(152), (91), (76), (30), (24), (24), (12), (2), (1), (1)
Host
Rabbit
(140), (30)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(134), (64), (51), (36), (20), (20), (14), (13), (12), (8), (3), (3), (2), (2), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mL
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Catalog No. ABIN189672
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Immunogen A synthetic peptide corresponding to residues in the C-term of human PI3-K 85 alpha was used as an immunogen.
Clone EP380Y
Isotype IgG
Purification Tissue culture supernatant
Alternative Name PIK3R1 / GRB1
Background PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signaltransduction. PI3-K consists of two subunits, the regulatory subunit p85 and the catalyticsubunit p110 (1-2). In quiescent cells p85 maintains p110 in a low activity state. Upongrowth factor stimulation, p85 directly interacts with phosphotyrosine residues ofactivated growth factor receptors or adaptor proteins leading to activation of p110. p85exists in two isoforms: p85, including two short forms termed p50 and p55/AS53 andp85 including short form p55PIK/p55 (3). The p85 adaptor protein uses the inter-SH2(iSH2) region to bind p110 and employs SH3 and SH2 domains, phosphotyrosineresidues, and proline-rich motifs to interact with activated tyrosine kinases such asBCR/ABL and NPM/ALK or adaptor proteins including Shc, c-Cbl, Crk-L, and Gab2(4-5). Alternate Names: anti-GRB1 antibody, anti-p50 alpha antibody, anti-p55 alpha antibody, anti-p85 alphaantibody, anti-Phosphatidylinositol 3 kinase associated p85 alpha antibody,anti-Phosphatidylinositol 3 kinase regulatory 1 antibody, anti-Phosphatidylinositol 3kinase regulatory alpha subunit antibody, anti-Phosphatidylinositol 3 kinase regulatorysubunit alpha antibody, anti-Phosphatidylinositol 3 kinase regulatory subunit polypeptide1 antibody, anti-Phosphoinositide 3 kinase regulatory subunit 1 antibody,anti-Phosphoinositide 3 kinase regulatory subunit polypeptide 1 (p85 alpha) antibody,anti-PI3 kinase p85 alpha subunit antibody, anti-PI3 kinase p85 subunit alpha antibody,anti-PI3K antibody, anti-PIK3R1 antibody, anti-PtdIns 3 kinase p85 alpha antibody.
Gene Symbol: PIK3R1
Gene ID 5295, 18708, 25513
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 85kDa.
Recommended dilutions: Flow Cytometry 1:60, Immunocytochemistry/Immunofluorescence 1:250-500, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:100-250, Immunoprecipitation 1:50, Western Blot 1:1000-10000
Protocol Protocol specific for PI 3 Kinase p85 alpha Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
anti-PI3 Kinase P85 alpha (C-Term) antibody Immunofluorescent staining of HeLa cells using anti- PI3-K p85 subunit alpha RabMAb (cat. #110-57350).
anti-PI3 Kinase P85 alpha (C-Term) antibody (2) Western blot analysis of anti- PI3-K p85 subunit alpha RabMAb (cat. # 110-57350), dilution 1:2,000. A: A431 B: MCF-7 C: Raji
anti-PI3 Kinase P85 alpha (C-Term) antibody (3) Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-PI3-K p85 subunit alpha RabMAb (cat. #110-57350).
General Jain, Langdon, Varticovski: "Tyrosine phosphorylation of p120cbl in BCR/abl transformed hematopoietic cells mediates enhanced association with phosphatidylinositol 3-kinase." in: Oncogene, Vol. 14, Issue 18, pp. 2217-28, 1997 (PubMed).

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