PI3 Kinase P85 alpha (C-Term) antibody

Antigen: PI3 Kinase P85 alpha

Binding Site: C-Term

Clonality: Monoclonal (EP380Y)

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN189672

Additional Information

Alternative name: PIK3R1 / GRB1

Gene ID: 5295, 18708, 25513

UniProt: P27986

Cross-Reactivity: Human, Rat (Rattus)

Isotype: IgG

Clone: EP380Y

Description: PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signaltransduction. PI3-K consists of two subunits, the regulatory subunit p85 and the catalyticsubunit p110 (1-2). In quiescent cells p85 maintains p110 in a low activity state. Upongrowth factor stimulation, p85 directly interacts with phosphotyrosine residues ofactivated growth factor receptors or adaptor proteins leading to activation of p110. p85exists in two isoforms: p85, including two short forms termed p50 and p55/AS53 andp85 including short form p55PIK/p55 (3). The p85 adaptor protein uses the inter-SH2(iSH2) region to bind p110 and employs SH3 and SH2 domains, phosphotyrosineresidues, and proline-rich motifs to interact with activated tyrosine kinases such asBCR/ABL and NPM/ALK or adaptor proteins including Shc, c-Cbl, Crk-L, and Gab2(4-5). Alternate Names: anti-GRB1 antibody, anti-p50 alpha antibody, anti-p55 alpha antibody, anti-p85 alphaantibody, anti-Phosphatidylinositol 3 kinase associated p85 alpha antibody,anti-Phosphatidylinositol 3 kinase regulatory 1 antibody, anti-Phosphatidylinositol 3kinase regulatory alpha subunit antibody, anti-Phosphatidylinositol 3 kinase regulatorysubunit alpha antibody, anti-Phosphatidylinositol 3 kinase regulatory subunit polypeptide1 antibody, anti-Phosphoinositide 3 kinase regulatory subunit 1 antibody,anti-Phosphoinositide 3 kinase regulatory subunit polypeptide 1 (p85 alpha) antibody,anti-PI3 kinase p85 alpha subunit antibody, anti-PI3 kinase p85 subunit alpha antibody,anti-PI3K antibody, anti-PIK3R1 antibody, anti-PtdIns 3 kinase p85 alpha antibody.

Specificity: A synthetic peptide corresponding to residues in the C-term of human PI3-K 85 alphawas used as an immunogen. Species Reactivity: Cross reacts in Human and Rat. Does not react in Mouse.

Application Details

Application Notes: Suggested working dilutions: flow cytometry / FACS analysis 1:60,immunohistochemistry 1:100-250,immunoprecipitation 1:50,Western Blot 1:500-2000,Immunocytochemistry 1:250-500

Purification: Aff - Purified

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. Preservative: 0.01% sodium azide.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Images

Western blot analysis of anti- PI3-K p85 subunit alpha RabMAb (cat. # 110-57350), dilution 1:2,000. A: A431 B: MCF-7 C: Raji

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-PI3-K p85 subunit alpha RabMAb (cat. #110-57350).

Immunofluorescent staining of HeLa cells using anti- PI3-K p85 subunit alpha RabMAb (cat. #110-57350).

Publications

Jain, Langdon, Varticovski: "Tyrosine phosphorylation of p120cbl in BCR/abl transformed hematopoietic cells mediates enhanced association with phosphatidylinositol 3-kinase." in: Oncogene, Vol. 14, Issue 18, pp. 2217-28, 1997 (PubMed).