You are viewing an incomplete version of our website. Please click to reload the website as full version.

ABCA1 antibody (ATP-Binding Cassette, Sub-Family A (ABC1), Member 1) (AA 1100-1300)

Details for Product anti-ABCA1 Antibody No. ABIN189744, Supplier: Log in to see
Antigen
  • ABC-1
  • ABC1
  • Abc1
  • ABCA1
  • CERP
  • HDLDT1
  • TGD
Epitope
AA 1100-1300
20
18
14
13
12
11
6
5
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Hamster, Human, Mouse (Murine), Mustelid, Pig (Porcine), Rat (Rattus)
120
111
69
18
15
13
13
13
12
Host
Rabbit
84
43
25
4
Clonality
Polyclonal
Conjugate
This ABCA1 antibody is un-conjugated
14
7
6
6
6
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
Application
Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry (FACS), Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
89
58
43
39
33
24
22
22
16
15
3
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen Partial peptide sequence (within residues 1100-1300) of human ABCA1 [UniProt# O95477]. Actual immunogen sequence is proprietary information.
Cross-Reactivity (Details) Canine reactivity reported in scientific literature (PMID: 24612239)
Purification Immunogen affinity purified
Alternative Name ABCA1 (ABCA1 Antibody Abstract)
Background Gene Symbol: ABCA1
Molecular Weight Theoretical MW: 220 kDa
Gene ID 19
UniProt O95477
Application Notes Western Blot 1:500, Simple Western 1:50, Chromatin Immunoprecipitation, Flow Cytometry 1:400, ELISA, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:200, Immunohistochemistry-Frozen, Gel Supershift Assay
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for ABCA1 Antibody Western BlotThe experiment was performed by treating RAW macrophages with 9-cis-retinoic acid and 22R-hydroxycholesterol, known inducers of ABCA1 expression in macrophages. Then total cell post-nuclear lysate (40ug protein) was separated by SDS-PAGE and detected using a 1:1000 dilution of NB 400-105 affinity purified Lot G incubated for 1 hour at room temperature (Lane A). Although there are lower molecular weight bands on the blot, the ABCA1 signal is excellent and gives the expected 3 bands. It is not known why ABCA1 runs as three bands, but it has been found to do so by many researchers. It is probably due to protein modifications such as glycosylation. The antibody was also tested against ABCA1 transiently expressed in 293 cells as an independent test with excellent results.NOTE: An important factor in detecting ABCA1 is in the cell type used. ABCA1 is expressed in very low levels in most cell types. Therefore, ABCA1 expression needs to be induced by using 22-hydroxycholesterol and 9-cis-retinoic acid as ligands for the transcription factor LXR.
1. Without heating at all (leave at room temp for about 15 to 20 minutes with Beta-mercaptoethanol), load 40 µg post-nuclear lysates* to 7.5 % or 4-15 % Tris-HCL SDS gel (Bio-RAD) in sample buffer. Do NOT boil the samples. (NP-40 will not interfere with the running of the protein on SDS-PAGE.)
. Transfer to nitrocellulose membrane at 100V 1hr or 30V overnight.
. Block membrane in 5 % milk in TBS-T for at least 1 hr. Wash with TBS-T 5 minutes.
. Blot with anti-ABCA1 antibody in 3 % milk in TBS-T for 1 hour.
. Wash with TBS-T 3 times, 10 minutes each.
. Blot with donkey anti-rabbit secondary, antibody (Amersham) at 1:2000 in 3 % milk in TBS-T for 1 hour.
. Wash with TBS-T 3 times, 10 minutes each.
. Detect with chemiluminescent reagent (Pierce).
. Expose to X-ray film, 30 sec, 1 min, and 3 min. TBS-T: Tris-buffered-saline with Tween-20See also the specific references mentioned in the datasheet. *Post-nuclear lysate is the result of sonication or dounce homogenization of lysate, centrifugation at low-speed, and the removal of nuclei. The resulting supernatant is called post-nuclear and contains cytosolic and membrane proteins without any of the nuclear components.IHC-FFPE sectionsI. Deparaffinization: A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous Peroxidase: A. Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 mL of Quenching Solution: -Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. -Use within 4 hours of preparation B. Place slides in distilled water: 2 changes for 2 minutes each. III. Retrieve Epitopes: A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-ABCA1 antibody (ATP-Binding Cassette, Sub-Family A (ABC1), Member 1) (AA 1100-1300) (ABIN189744) Western Blot analysis of ABCA1, using ABIN152883. Samples: 40 ug of total cell post-n...
Western Blotting (WB) image for anti-ABCA1 antibody (ATP-Binding Cassette, Sub-Family A (ABC1), Member 1) (AA 1100-1300) (ABIN189744) Detection of ABCA1 in mouse peritoneal macrophages using ABIN152883 (Lot L). ECL expo...
Immunohistochemistry (IHC) image for anti-ABCA1 antibody (ATP-Binding Cassette, Sub-Family A (ABC1), Member 1) (AA 1100-1300) (ABIN189744) anti-ATP-Binding Cassette, Sub-Family A (ABC1), Member 1 (ABCA1) (AA 1100-1300) antibody (Image 3)
Western Blotting (WB) image for anti-ABCA1 antibody (ATP-Binding Cassette, Sub-Family A (ABC1), Member 1) (AA 1100-1300) (ABIN189744) Western Blot: ABCA1 Antibody [ABIN189744] - Detection of ABCA1 in mouse peritoneal ma...
Flow Cytometry (FACS) image for anti-ABCA1 antibody (ATP-Binding Cassette, Sub-Family A (ABC1), Member 1) (AA 1100-1300) (ABIN189744) Flow Cytometry: ABCA1 Antibody [ABIN189744] - ABCA-1 FITC conjugated antibody of huma...
Flow Cytometry (FACS) image for anti-ABCA1 antibody (ATP-Binding Cassette, Sub-Family A (ABC1), Member 1) (AA 1100-1300) (ABIN189744) Flow Cytometry: ABCA1 Antibody [ABIN189744] - ABCA1 antibody was tested at 1: 400 in ...
Immunofluorescence (IF) image for anti-ABCA1 antibody (ATP-Binding Cassette, Sub-Family A (ABC1), Member 1) (AA 1100-1300) (ABIN189744) Immunocytochemistry/Immunofluorescence: ABCA1 Antibody [ABIN189744] - ABCA1 antibody ...
Product cited in: Sallam, Ito, Rong, Kim, van Stijn, Chamberlain, Jung, Chao, Jones, Gilliland, Wu, Su, Tangirala, Tontonoz, Hong: "The macrophage LBP gene is an LXR target that promotes macrophage survival and atherosclerosis." in: Journal of lipid research, Vol. 55, Issue 6, pp. 1120-30, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Merkl, Ertl, Handschuh, Aurich, Schäfer-Somi: "The cholesterol transporter ABCA1 is expressed in stallion spermatozoa and reproductive tract tissues." in: Theriogenology, Vol. 85, Issue 6, pp. 1080-9, 2016 (PubMed).

Fournier, Tardivel, Benoist, Vedie, Rousseau-Ralliard, Nowak, Allaoui, Paul: "Eicosapentaenoic acid membrane incorporation impairs ABCA1-dependent cholesterol efflux via a protein kinase A signaling pathway in primary human macrophages." in: Biochimica et biophysica acta, Vol. 1861, Issue 4, pp. 331-41, 2016 (PubMed). Further details: Western Blotting

Schäfer-Somi, Palme: "Seminal Plasma Characteristics and Expression of ATP-binding Cassette Transporter A1 (ABCA1) in Canine Spermatozoa from Ejaculates with Good and Bad Freezability." in: Reproduction in domestic animals = Zuchthygiene, Vol. 51, Issue 2, pp. 232-9, 2016 (PubMed). (Sample species: Dog (Canine)). Further details: Immunocytochemistry,Immunofluorescence

Shrestha, Hussein, Savas, Ouimet, Barrett, Leone, Yates, Moore, Fisher, Garabedian: "Poly(ADP-ribose) Polymerase 1 Represses Liver X Receptor-mediated ABCA1 Expression and Cholesterol Efflux in Macrophages." in: The Journal of biological chemistry, Vol. 291, Issue 21, pp. 11172-84, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Ollikainen, Tulamo, Lehti, Lee-Rueckert, Hernesniemi, Niemelä, Ylä-Herttuala, Kovanen, Frösen: "Smooth Muscle Cell Foam Cell Formation, Apolipoproteins, and ABCA1 in Intracranial Aneurysms: Implications for Lipid Accumulation as a Promoter of Aneurysm Wall Rupture." in: Journal of neuropathology and experimental neurology, Vol. 75, Issue 7, pp. 689-99, 2016 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Kemmerer, Wittig, Richter, Brüne, Namgaladze: "AMPK activates LXRα and ABCA1 expression in human macrophages." in: The international journal of biochemistry & cell biology, Vol. 78, pp. 1-9, 2016 (PubMed). Further details: Western Blotting

Hsieh, Koseki, Molusky, Yakushiji, Ichi, Westerterp, Iqbal, Chan, Abramowicz, Tascau, Takiguchi, Yamashita, Welch, Di Paolo, Hussain, Lefkowitch, Rader, Tall: "TTC39B deficiency stabilizes LXR reducing both atherosclerosis and steatohepatitis." in: Nature, Vol. 535, Issue 7611, pp. 303-7, 2016 (PubMed).

Hnatiuk, Ong, Olea, Locatelli, Riegler, Lee, Jen, De Lorenzi, Giménez, Laguens, Wu, Crottogini: "Allogeneic Mesenchymal Stromal Cells Overexpressing Mutant Human Hypoxia-Inducible Factor 1-α (HIF1-α) in an Ovine Model of Acute Myocardial Infarction." in: Journal of the American Heart Association, Vol. 5, Issue 7, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Zinkhan, Zalla, Carpenter, Yu, Yu, Chan, Joss-Moore, Lane: "Intrauterine growth restriction combined with a maternal high-fat diet increases hepatic cholesterol and low-density lipoprotein receptor activity in rats." in: Physiological reports, Vol. 4, Issue 13, 2016 (PubMed).

Kim, Lim, Yoon, Ahn, Park, Kim, Kang: "Liver X receptor and STAT1 cooperate downstream of Gas6/Mer to induce anti-inflammatory arginase 2 expression in macrophages." in: Scientific reports, Vol. 6, pp. 29673, 2016 (PubMed).

Khan, Ahmed, Pushparaj, Abuzenadah, Kumosani, Barbour, AlQahtani, Gauthaman: "Ajwa Date (Phoenix dactylifera L.) Extract Inhibits Human Breast Adenocarcinoma (MCF7) Cells In Vitro by Inducing Apoptosis and Cell Cycle Arrest." in: PLoS ONE, Vol. 11, Issue 7, pp. e0158963, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Cui, Ye, Chopp, Venkat, Zacharek, Yan, Ning, Yu, Cui, Chen: "miR-145 Regulates Diabetes-Bone Marrow Stromal Cell-Induced Neurorestorative Effects in Diabetes Stroke Rats." in: Stem cells translational medicine, Vol. 5, Issue 12, pp. 1656-1667, 2016 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry

de Gaetano, Crean, Barry, Belton: "M1- and M2-Type Macrophage Responses Are Predictive of Adverse Outcomes in Human Atherosclerosis." in: Frontiers in immunology, Vol. 7, pp. 275, 2016 (PubMed). Further details: Western Blotting

Shi, Mao, Zhong, Liu, Zhao, Yu, Zhu, Wei, Zhu, Sun, Mao, Zeng: "Lanatoside C Promotes Foam Cell Formation and Atherosclerosis." in: Scientific reports, Vol. 6, pp. 20154, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Hong, Xu, Huang, Lei, Xu, Zhao, Yu: "Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency." in: Scientific reports, Vol. 6, pp. 20503, 2016 (PubMed). (Sample species: Pig (Porcine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Leng, Iwanowycz, Saaoud, Wang, Wang, Sergin, Razani, Fan: "Ursolic acid enhances macrophage autophagy and attenuates atherogenesis." in: Journal of lipid research, Vol. 57, Issue 6, pp. 1006-16, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Zhang, Hu, Zhong, Wang, Yang, Liu, Li, Wang, Zhou, Zhang, Xu, Bu, Zhuang: "Quercetin stabilizes apolipoprotein E and reduces brain Aβ levels in amyloid model mice." in: Neuropharmacology, Vol. 108, pp. 179-92, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Lai, Azzam, Lin, Rai, Lowe, Gabor, Madenspacher, Aloor, Parks, Näär, Fessler: "MicroRNA-33 Regulates the Innate Immune Response via ATP Binding Cassette Transporter-mediated Remodeling of Membrane Microdomains." in: The Journal of biological chemistry, Vol. 291, Issue 37, pp. 19651-60, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Bechor, Zolberg Relevy, Harari, Almog, Kamari, Ben-Amotz, Harats, Shaish: "9-cis β-Carotene Increased Cholesterol Efflux to HDL in Macrophages." in: Nutrients, Vol. 8, Issue 7, 2016 (PubMed).

Did you look for something else?