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MLX Interacting Protein-Like (MLXIPL) (C-Term), (AA 800-852), (Isoform 1) antibody

Details for Product No. ABIN189747
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Antigen
Synonyms ChREBP, MLXIPL, CHREBP, MIO, MONDOB, WBSCR14, WS-bHLH, bHLHd14, Wbscr14
Epitope
C-Term, AA 800-852, Isoform 1
(18), (13), (9), (7), (6), (3), (2), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(47), (24), (23), (13), (12), (12), (12)
Host
Rabbit
(48), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(33), (21), (16), (10), (10), (9), (8), (3), (3)
Pubmed 4 references available
Quantity 0.025 mL
Shipping to United States (Change)
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Catalog No. ABIN189747
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Immunogen A C-terminal synthetic peptide made to the human CHREBP protein sequence (between residues 800-852). Isoform 1/Alpha.
Purification affinity purified
Alternative Name MLXIPL
Background ChREBP is a transcription factor involved in activating genes that encode enzymes offatty acid biosynthesis in liver and adipose. This protein may have relevance to lipidmetabolism, obesity, and type II diabetes. Alternate Names: anti-WBSCR14 antibody, anti-Williams Beuren Syndrome Chromosome Region 14antibody, anti-MIO antibody, anti-MONDOB antibody, anti-WS-bHLH antibody. Related Diseases: Williams-Beuren syndrome, Cancer
Gene Symbol: MLXIPL
Gene ID 51085
UniProt Q9NP71
Application Notes This CHREBP antibody is useful in ChIP (PMID: 21282101), Immunohistochemistry paraffin embedded sections, Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~95 kDa.
Recommended dilutions: Chromatin Immunoprecipitation 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:100-1:500, Immunohistochemistry 1:100, Immunohistochemistry-Paraffin 1:100, Immunoprecipitation 1:10-1:500, Western Blot 1:1000
Protocol Protocol specific for CHREBP Antibody Procedure for Western blot
1. Wash human hepatocytes grown in a culture dish with PBS twice and lyse the cells by Pierce M-Per reagent.
. Measure protein concentration in lysates by Bio-rad protein assay.
. Mix 20 µg of protein with Bio-rad loading buffer and 5 % 2-mercaptoethanol. Boil for 5 minutes.
. Place Bio-rad 4-15 % Tris-Hcl gel. Load and run at 125V for 1 hour.
. Transfer the proteins to nitrocellulose membrane. Run at 100V for 1 hour.
. Block the membrane by 1 % nonfat dry milk in Tris-buffered saline-Tween (TBS-T) for 1 hour.
. Rinse the membrane twice by TBS-T.
. Incubate with rabbit anti-ChREBP antibody 1:1,000-1:3,000 in TBS-T with 0.02 % BSA overnight at 4 degrees C.
. Wash the membrane twice by TBS-T.
. Incubate with Goat anti-rabbit AP conjugated antibody 1:3000 in TBS-T for 1 hour at room temperature.
. Wash the membrane twice by TBS-T.
. Detect the signal by Bio-rad Immun-star kit and develop film.Procedure for immunofluorescent staining
. Wash human hepatocytes grown on cover-slips with PBS twice.
. Fix cells with 10 % buffered formalin for 25 minutes at room temperature.
. Wash cells with PBS 3 times.
. Incubate with 0.5 % Triton X-100 in PBS for 5 minutes.
. Wash cells with PBS 3 times.
. Block with 1 % BSA in PBS for 15 minutes at 37 degrees C.
. Incubate with rabbit anti-ChREBP antibody 1:100-1:500 in PBS with 1 % BSA for 30 minutes at 37 degrees C.
. Wash cells with PBS 3 times.
. Incubate with Goat anti-rabbit Alexa Fluor 568 antibody 1:2,000 in PBS with 1 % BSA for 30 minutes at 37 degrees C.
. Wash cells with PBS 3 times.
. Mount by Vectashield mounting medium with DAPI.
. Take pictures by using Rhodamine and DAPI filters.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-citrate/Phosphate, pH 7-8, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
General Xu, Christian, Jump: "Regulation of rat hepatic L-pyruvate kinase promoter composition and activity by glucose, n-3 polyunsaturated fatty acids, and peroxisome proliferator-activated receptor-alpha agonist." in: The Journal of biological chemistry, Vol. 281, Issue 27, pp. 18351-62, 2006 (PubMed).

Wang, Botolin, Xu et al.: "Regulation of hepatic fatty acid elongase and desaturase expression in diabetes and obesity." in: Journal of lipid research, Vol. 47, Issue 9, pp. 2028-41, 2006 (PubMed).

Dentin, Benhamed, Hainault et al.: "Liver-specific inhibition of ChREBP improves hepatic steatosis and insulin resistance in ob/ob mice." in: Diabetes, Vol. 55, Issue 8, pp. 2159-70, 2006 (PubMed).

Erhuma, Salter, Sculley et al.: "Prenatal exposure to a low-protein diet programs disordered regulation of lipid metabolism in the aging rat." in: American journal of physiology. Endocrinology and metabolism, Vol. 292, Issue 6, pp. E1702-14, 2007 (PubMed).

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