GPC1 antibody (Internal Region)

Catalog No. ABIN1899462

Product Details anti-GPC1 Antibody

Target: GPC1 (Glypican 1 (GPC1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: This antibody is specific for human glypican-1 protein. A BLAST analysis was used to suggest partial cross-reactivity with glypican from rat, mouse, Macaque, dog, cattle, and opossum sources based on 100 - 88 % homology with the immunizing sequence. Cross-reactivity with glypican from other sources has not been determined.

Purification: Protein A purified

Sterility: Sterile filtered

Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human glypican-1 protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:50000), IHC, IHC-P (10 μg/mL), WB (1:500 - 1:2000)

Usage: This protein A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~61 kD in size corresponding to glypican by western blotting in the appropriate cell lysate or extract. The higher molecular weight (110kD) of transfected Fc-glypican compared with the expected MW of glypican is likely due to the presence of the Fc-tag.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide, sterile filtered

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for GPC1

Target: GPC1 (Glypican 1 (GPC1))

Alternative Name: GPC1 / Glypican (GPC1 Products)

Synonyms: gpc1 antibody, zgc:63947 antibody, GPC1 antibody, Glypican-1 antibody, glypican antibody, AI462976 antibody, HSPG M12 antibody, HSPGM12 antibody, glypican 1b antibody, glypican 1 antibody, gpc1b antibody, GPC1 antibody, gpc1 antibody, Gpc1 antibody

Background: Name/Gene ID: GPC1

Synonyms: GPC1, Glypican 1, Glypican, Glypican-1, Glypican proteoglycan 1

Gene ID: 2817

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation