This Superoxide dismutase copper chaperone antibody is un-conjugated
Application
Western Blotting (WB)
Cross-Reactivity (Details)
Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
Predicted Reactivity
dicots including: Glycine max, Solanum tuberosum
Characteristics
Expected / apparent Molecular Weight of the Antigene: 33.8 / 37 kDa (Arabidopsis thaliana)
Purification
serum
Immunogen
The antibody was raised to the Arabidopsis thaliana full length CCs protein Q4ZJI5, fused to His-tag. Overexpressed using pET28a vector in Bl21 E. coli (Cd+) and purified using His-column. After purification, the His tag was cleaved using thrombin and then theprotein was purified using Resource-Q column connected to HPLC.
CCS
Reactivity: Human
ELISA, IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
1 : 1000 with alkaline phosphatase (WB)
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 200 μL of sterile water.
Handling Advice
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Storage
-20 °C
Storage Comment
store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
AGI Code: At1g12520 Copper is an essential element for function of chloroplasts. It is a co-factor for superoxide dismutase (SOD) and for plastocyanin. Copper is transported to chloroplasts with a help of Copper Chaperones, which carry out the delivery of copper from transporters to targets. In the last step chaperone binds to a copper dependent enzyme and inserts the copper ions into its active site. The Arabidopsis thaliana gene AtCCS encodes a copper chaperone for SOD. The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD.