Thioredoxin Reductase antibody (N-Term)

Catalog No. ABIN190746

Product Details anti-Thioredoxin Reductase Antibody

Target: Thioredoxin Reductase (TrxR)

Binding Specificity: N-Term

Reactivity: Chlamydomonas reinhardtii

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Thioredoxin Reductase antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity (Details): Not reactive in: Arabidopsis thaliana

Predicted Reactivity: Chlamydia sp. Ostreococcus luminarius, Physcomitrella patens, Synechococcus sp.

Characteristics: Expected / apparent Molecular Weight of the Antigene: 55.3 kDa

Purification: serum

Immunogen: KLH-conjugated peptide derived from N-terminal domain of NtrC of Chlamydomonas reinhardtii A8HNQ7

Application Details

Application Notes: 1 : 500 with standard ECL (WB)

Comment:

antibody will react with long NtrC of Chlamydomonas reinhardtii proteins and shorter NtrA-type rpoteins, which would be distinguished by migration size.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water.

Buffer: PBS pH 7.4

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for Thioredoxin Reductase

Target: Thioredoxin Reductase (TrxR)

Alternative Name: Thioredoxin reductase (TR/TRxR) (NtrC) (TrxR Products)

Synonyms: Prx V antibody, peroxiredoxin 5 antibody, thioredoxin reductase antibody, thioredoxin-disulfide reductase antibody, PRDX5 antibody, trxR antibody, SACI_RS04445 antibody

Background: Thioredoxin Reductase (TR, TrxR) is the only known enzyme (EC 1.8.1.9) which is reducing thoredoxin (Trx). Activity of thoredoxin is essential for growth and survival of the cell. There seem to be one isoform of NtrC protein in Arabidopsis which includes an N-terminal reductase domain and a C-terminal domain related to thioredoxin proteins. Arabidopsis and Chlmydomonas NtrC proteins are ca. 568 amino acids long, but include ca. 80 amino acid signal peptides, for mature protein size of ca. 488 amino acids.

Molecular Weight: 55.3 kDa

UniProt: A8HNQ7