Ide (mouse) antibody

Antigen: Ide (mouse)

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190754

Additional Information

Gene ID: 15925 (mouse), 25700 (rat)

Immunogen: C-QQYNYDRDNIE

Format: Affinity-purified

Description: All names and symbols: Ide, insulin-degrading enzyme, 1300012G03Rik, 4833415K22Rik, AA675336, AI507533, INSULYSIN, insulinase, insulysin. Official Symbol: Ide. RefSeq number(s): NP_112419.2

Specificity: Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 110kDa band observed in Mouse Brain and Rat Brain lysates (calculated MW of 118kDa according to NP_112419.2). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190754 (1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Zhao, Xiang, Haroutunian et al.: "Insulin degrading enzyme activity selectively decreases in the hippocampal formation of cases at high risk to develop Alzheimer's disease." in: Neurobiology of aging, Vol. 28, Issue 6, pp. 824-30, 2007 (PubMed).