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Insulin-Degrading Enzyme (IDE) (Internal Region) antibody

Details for Product No. ABIN190754
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Antigen
Synonyms INSULYSIN, AA675336, AI507533, 1300012G03Rik, 4833415K22Rik, INSDEGM, zgc:162603, IDE
Epitope
Internal Region
(16), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus)
(66), (35), (25), (25), (25), (24), (4)
Host
Goat
(42), (15), (14)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(48), (27), (19), (14), (11), (9), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
Options
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Availability Available
Catalog No. ABIN190754
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence C-QQYNYDRDNIE, from the internal region of the protein sequence according to NP_112419.2.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Ide
Background Synonyms: Ide, insulin-degrading enzyme, 1300012G03Rik, 4833415K22Rik, AA675336, AI507533, INSULYSIN, insulinase, insulysin
Gene ID 15925, 25700
NCBI Accession NP_112419.2
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 110 kDa band observed in Mouse Brain and Rat Brain lysates (calculated MW of 118 kDa according to NP_112419.2). Recommended concentration: 1-3 µg/mL. An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunising peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Insulin-Degrading Enzyme (IDE) (Internal Region) antibody ABIN190754 (1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Zhao, Xiang, Haroutunian et al.: "Insulin degrading enzyme activity selectively decreases in the hippocampal formation of cases at high risk to develop Alzheimer's disease." in: Neurobiology of aging, Vol. 28, Issue 6, pp. 824-30, 2007 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN190754
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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