Insulin-Degrading Enzyme (IDE) (Internal Region) antibody

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Antigen
Synonyms zgc:162603, IDE, 1300012G03Rik, 4833415K22Rik, AA675336, AI507533, INSDEGM, INSULYSIN
Epitope
Internal Region
(33), (13), (13), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus)
(105), (42), (33), (6), (3), (3), (2), (2), (2), (1)
Host
Goat
(71), (40), (9)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (5), (5), (4), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(95), (49), (37), (24), (13), (4), (3), (2), (2), (2), (1)
Pubmed 1 reference available
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Quantity 100 μg
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Immunogen Peptide with sequence C-QQYNYDRDNIE, from the internal region of the protein sequence according to NP_112419.2.
Blocking Peptide Blocking peptide for this product available: Insulin-Degrading Enzyme (IDE) peptide (ABIN369686)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Ide (IDE Antibody Abstract)
Background Synonyms: Ide, insulin-degrading enzyme, 1300012G03Rik, 4833415K22Rik, AA675336, AI507533, INSULYSIN, insulinase, insulysin
Gene ID 15925, 25700
NCBI Accession NP_112419
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 110 kDa band observed in Mouse Brain and Rat Brain lysates (calculated MW of 118 kDa according to NP_112419.2). Recommended concentration: 1-3 µg/mL. An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunising peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Image no. 1 for anti-Insulin-Degrading Enzyme (IDE) (Internal Region) antibody (ABIN190754) ABIN190754 (1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Pri...
Background publications Zhao, Xiang, Haroutunian et al.: "Insulin degrading enzyme activity selectively decreases in the hippocampal formation of cases at high risk to develop Alzheimer's disease." in: Neurobiology of aging, Vol. 28, Issue 6, pp. 824-30, 2007 (PubMed).

Catalog No. ABIN190754
$221.76
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