FH antibody (Internal Region)

Catalog No. ABIN190758

Product Details anti-FH Antibody

Target: FH (Fumarate Hydratase (FH))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)

Purpose: Fumarase / FH

Sequence: HPNDHVNKSQ SSND

Predicted Reactivity: Human, Mouse, Rat, Dog

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-HPNDHVNKSQSSND, from the internal region of the protein sequence according to NP_000134.2.

Isotype: IgG

Application Details

Application Notes:

DS WB Results: Approx 48 kDa band observed in Human Kidney, Liver and Lung lysates (calculated MW of 54.6 kDa according to NP_000134.2). The observed molecular weight corresponds to earlier findings with antibodies from different sources. In transfected HEK293 transiently expressing Fumarase a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.01-0.03 μg/mL.

DS IHC Results: Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 3.75 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant Fumarase captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FH

Target: FH (Fumarate Hydratase (FH))

Alternative Name: Fumarate Hydratase (FH Products)

Background: Fumarate hydratase, HLRCC, LRCC, MCL, MCUL1, fumarase, leiomyomatosis and renal cell cancer, multiple hereditary cutaneous leiomyomata

Gene ID: 2271

NCBI Accession: NP_000134