Peroxisome Proliferator-Activated Receptor Gamma, Coactiv (PPARGC1A) antibody

Antigen: Peroxisome Proliferator-Activated Receptor Gamma, Coactiv (PPARGC1A)

Synonyms: LEM6, PGC1, PGC1A, PGC-1v, PPARGC1, PGC-1(alpha), AVPGC-1ALPHA

Clonality: Polyclonal

Host: Goat

Reactivity: Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190764

Additional Information

Alternative name: PGC1A

Gene ID: 10891, 19017 (mouse), 83516 (rat)

Immunogen: C-DGLFDDSEDESDK

Format: Affinity-purified

Description: All names and symbols: PGC1A, PPARGC1A, peroxisome proliferator-activated receptor gamma, coactivator 1 alpha, LEM6, PGC-1(alpha), PGC-1v, PGC1, PPARGC1, PPAR gamma coactivator variant form, PPAR gamma coactivator-1, ligand effect modulator-6, peroxisome proliferative activated receptor, gamma, coactivator 1, peroxisome proliferative activated receptor, gamma, coactivator 1, alpha. Official Symbol: PPARGC1A. RefSeq number(s): NP_037393.1

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 90kDa band observed in Rat Stomach lysates (calculated MW of 90.6kDa according to Rat NP_112637.1 and 91.0kDa according to Human NP_037393.1). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190764 (0.1µg/ml) staining of Rat Stomach lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Handschin, Kobayashi, Chin et al.: "PGC-1alpha regulates the neuromuscular junction program and ameliorates Duchenne muscular dystrophy." in: Genes & development, Vol. 21, Issue 7, pp. 770-83, 2007 (PubMed).