Calcium Channel, Voltage-Dependent, alpha 2/delta Subunit 1 (CACNA2D1) antibody

Antigen: Calcium Channel, Voltage-Dependent, alpha 2/delta Subunit 1 (CACNA2D1)

Synonyms: CACNA2, CCHL2A, CACNL2A, CACNA2D1, DKFZp469K2211

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190770

Additional Information

Alternative name: CACNA2D1

Gene ID: 781, 12293 (mouse), 25399 (rat)

Immunogen: C-QEIFNKYNKDKKVR

Format: Affinity-purified

Description: All names and symbols: CACNA2D1, calcium channel, voltage-dependent, alpha 2/delta subunit 1, CACNA2, CACNL2A, CCHL2A, MHS3, H_DJ0560O14.1, WUGSC:H_DJ0560O14.1, calcium channel, L type, alpha 2 polypeptide, dihydropyridine-sensitive L-type, calcium channel alpha-2/delta subunit. Official Symbol: CACNA2D1. RefSeq number(s): NP_000713.2

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 140kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 123kDa according to NP_000713.2). Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190770 (0.03µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Hullin, Matthes, von Vietinghoff et al.: "Increased expression of the auxiliary beta2-subunit of ventricular L-type Ca2+ channels leads to single-channel activity characteristic of heart failure." in: PLoS ONE, Vol. 2, Issue 3, pp. e292, 2007 (PubMed).

Neely, Hess, Costigan et al.: "A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene." in: Cell, Vol. 143, Issue 4, pp. 628-38, 2010 (PubMed).