Transglutaminase 2 antibody (Internal Region)

Catalog No. ABIN190773

Product Details anti-Transglutaminase 2 Antibody

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Transglutaminase 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: TGM2

Sequence: NFESDKLKAV KGFR

Specificity: This antibody is expected to recognise isoform a (NP_004604.2) only.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-NFESDKLKAVKGFR, from the internal region (near the C Terminus) of the protein sequence according to NP_004604.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta. Recommended concentration: 5-10 μg/mL.

Western Blot: Approx 90 kDa band observed in Human Lung lysates and approx 85 kDa in lysates of cell line A549 (calculated MW of 77.3 kDa according to NP_004604.2). Recommended concentration: 0.03-0.1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Transglutaminase 2

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Alternative Name: TGM2 (TGM2 Products)

Background: TGM2, transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase), TG2, TGC, C polypeptide, TGase C, TGase-H, protein-glutamine-gamma-glutamyltransferase, tissue transglutaminase, transglutaminase 2, transglutaminase C

Gene ID: 7052

NCBI Accession: NP_004604

Pathways: Tube Formation, Thromboxane A2 Receptor Signaling