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Interferon Induced with Helicase C Domain 1 (IFIH1) (Internal Region) antibody

Details for Product No. ABIN190791, Supplier: Log in to see
Antigen
  • MDA5
  • Hlcd
  • IDDM19
  • MDA-5
  • RLR-2
  • 9130009C22Rik
  • Helicard
  • RGD1560496
Epitope
Internal Region
18
18
10
7
6
4
3
3
3
1
1
1
1
1
1
Reactivity
Mouse (Murine)
107
41
4
2
1
Host
Goat
72
30
22
Clonality
Polyclonal
Conjugate
Un-conjugated
3
2
2
2
2
2
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
85
58
42
19
11
6
4
3
3
1
1
1
Supplier
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Immunogen Peptide with sequence C-KQKLRNNDLKKQN, from the internal region of the protein sequence according to NP_082111.1.
Blocking Peptide Blocking peptide for this product available: ABIN369345
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name MDA5 / IFIH1 (IFIH1 Antibody Abstract)
Background Synonyms: Ifih1, interferon induced with helicase C domain 1, 9130009C22Rik, Helicard, Hlcd, MDA-5, MDA5, MGC90959, melanoma differentiation associated protein-5
Gene ID 71586
NCBI Accession NP_082111
Pathways
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave an approx 80 kDa band in Mouse Brain lysates after 1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 116 kDa according to NP_082111.1. The 80 kDa band was successfully blocked by incubation with the immunizing peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Kato, Takeuchi, Sato et al.: "Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses." in: Nature, Vol. 441, Issue 7089, pp. 101-5, 2006 (PubMed).