Roundabout, Axon Guidance Receptor, Homolog 3 (Drosophila) (ROBO3) (internal) antibody

Antigen: Roundabout, Axon Guidance Receptor, Homolog 3 (Drosophila) (ROBO3)

Synonyms: HGPS, RIG1, HGPPS, RBIG1, FLJ21044, Rig1, Rbig1, Rig-1, Robo3a, Robo3b

Epitope: internal

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190793

Additional Information

Alternative name: ROBO3 / RIG1 (internal)

Gene ID: 64221

Immunogen: C-RRKQRKELSHYT

Format: Affinity-purified

Description: All names and symbols: ROBO3, RIG1, roundabout, axon guidance receptor, homolog 3 (Drosophila) , FLJ21044, HGPPS, RBIG1, retinoblastoma inhibiting gene 1, roundabout, axon guidance receptor, homolog 3. Official Symbol: ROBO3. RefSeq number(s): NP_071765.2

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 150kDa band observed in Human Brain (Cerebellum and Hippocampus) lysates (calculated MW of 148kDa according to NP_071765.2). Recommended concentration: 0.5-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190793 (0.5µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Kato, Takeuchi, Sato et al.: "Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses." in: Nature, Vol. 441, Issue 7089, pp. 101-5, 2006 (PubMed).

Bauer, Dowejko, Bosserhoff et al.: "Slit-2 facilitates interaction of P-cadherin with Robo-3 and inhibits cell migration in an oral squamous cell carcinoma cell line." in: Carcinogenesis, Vol. 32, Issue 6, pp. 935-43, 2011 (PubMed). (1:50).