PSME3 (PSME3) antibody

Antigen: PSME3 (PSME3)

Synonyms: Ki, PA28G, REG-GAMMA, PA28-gamma

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190797

Additional Information

Alternative name: PSME3

Gene ID: 10197, 19192 (mouse), 287716 (rat)

Immunogen: C-KNIEKIKRPRSSN

Format: Affinity-purified

Description: All names and symbols: PSME3, proteasome (prosome, macropain) activator subunit 3, Ki, PA28-gamma, PA28G, REG-GAMMA , 11S regulator complex gamma subunit, Ki antigen, Ki nuclear autoantigen, activator of multicatalytic protease subunit 3, proteasome activator 28-gamma, proteasome activator subunit 3. Official Symbol: PSME3. RefSeq number(s): NP_005780.2, NP_789839.1

Specificity: This antibody is expected to recognise both reported isoforms (NP_005780.2 and NP_789839.1). Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 132,000. Western Blot: Approx 30kDa band observed in lysates of cell lines Jurkat and K562 (calculated MW of 29.5kDa according to NP_005780.2 and 30.9kDa according to NP_789839.1). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Immunology, Proteolysis / Ubiquitin, Proteasome

Restrictions: For Research Use only

Images

ABIN190797 (0.1µg/ml) staining of K562 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Matsudaira, Takeuchi, Takasaki et al.: "Relationships between autoantibody responses to deletion mutants of Ki antigen and clinical manifestations of lupus." in: The Journal of rheumatology, Vol. 30, Issue 6, pp. 1208-14, 2003 (PubMed).