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FOXO4 antibody (Forkhead Box O4) (Internal Region)

Details for Product anti-FOXO4 Antibody No. ABIN190813, Supplier: Log in to see
Antigen
  • afx
  • AFX
  • AFX1
  • Afxh
  • FOXO4
  • foxo4
  • MLLT7
  • Mllt7
  • RGD1561201
Alternatives
anti-Human FOXO4 antibody for Western Blotting
Epitope
Internal Region
37
34
23
19
15
10
7
5
5
5
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
232
135
96
7
5
2
1
1
1
1
1
Host
Goat
212
14
7
Clonality
Polyclonal
Conjugate
This FOXO4 antibody is un-conjugated
6
6
6
3
3
3
3
3
3
3
3
3
3
3
3
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
173
88
74
33
30
29
18
7
5
Supplier
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Immunogen Peptide with sequence C-EAASQDRMPQDLD, from the internal region of the protein sequence according to NP_005929.2.
Blocking Peptide Blocking peptide for this product available: ABIN368950
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name FOXO4 / MLLT7 (FOXO4 Antibody Abstract)
Background Synonyms: MLLT7, FOXO4, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila) translocated to, 7, AFX, AFX1, MGC120490, myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog) translocated to, 7
Gene ID 4303
NCBI Accession NP_005929
Pathways Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 60-65 kDa band observed in lysates of cell line K562 (calculated MW of 53.7 kDa according to NP_005929.2). Recommended concentration: 1-3 µg/mL. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant FOXO4 captured by a rabbit antibody. Recommended reporter concentration: 0.5-1 µg/mL
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-FOXO4 antibody (Forkhead Box O4) (Internal Region) (ABIN190813) ABIN190813 (1µg/ml) staining of K562 cell lysate (35µg protein in RIPA buffer). Prima...
Background publications Paik, Kollipara, Chu, Ji, Xiao, Ding, Miao, Tothova, Horner, Carrasco, Jiang, Gilliland, Chin, Wong, Castrillon, DePinho: "FoxOs are lineage-restricted redundant tumor suppressors and regulate endothelial cell homeostasis." in: Cell, Vol. 128, Issue 2, pp. 309-23, 2007