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Neuroligin 2 antibody (Internal Region)

Verified NLGN2 Reactivity: Human WB, ELISA, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN190848
  • Target See all Neuroligin 2 (NLGN2) Antibodies
    Neuroligin 2 (NLGN2)
    Binding Specificity
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 10
    • 10
    • 9
    • 5
    • 5
    • 5
    • 5
    • 5
    • 1
    Human
    Host
    • 10
    • 1
    Goat
    Clonality
    • 11
    Polyclonal
    Conjugate
    • 7
    • 1
    • 1
    • 1
    • 1
    This Neuroligin 2 antibody is un-conjugated
    Application
    • 7
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    Neuroligin 2
    Sequence
    NPPDTDIRDP GKK
    Cross-Reactivity
    Dog, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-NPPDTDIRDPGKK, from the internal region of the protein sequence according to NP_065846.1.
    Isotype
    IgG
  • Application Notes

    Western Blot: Approx 90 kDa band observed in Human Pancrease lysates (calculated MW of 90.8 kDa according to NP_065846.1). Recommended concentration: 2-3 μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Mouse and

    Peptide ELISA: antibody detection limit dilution 1:4000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the mitochondria/cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentrati

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Zelano, Berg, Thams, Hailer, Cullheim: "SynCAM1 expression correlates with restoration of central synapses on spinal motoneurons after two different models of peripheral nerve injury." in: The Journal of comparative neurology, Vol. 517, Issue 5, pp. 670-82, (2009) (PubMed).

  • Target
    Neuroligin 2 (NLGN2)
    Alternative Name
    NLGN2 (NLGN2 Products)
    Synonyms
    Nlgn2 antibody, GB10066 antibody, NLGN2 antibody, CCE-L2 antibody, NV50031 antibody, Nv123771 antibody, NL2 antibody, RP23-422L16.24 antibody, neuroligin 2 antibody, LOC410779 antibody, NLGN2 antibody, LOC663865 antibody, LOC100123771 antibody, Nlgn2 antibody
    Background
    Neuroligin 2 , KIAA1366, NLGN2
    Gene ID
    57555, 216856, 117096
    NCBI Accession
    NP_065846
    Pathways
    Cell-Cell Junction Organization, Synaptic Membrane
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