Sortilin-Related VPS10 Domain Containing Receptor 1 (SORCS1) antibody

Antigen: Sortilin-Related VPS10 Domain Containing Receptor 1 (SORCS1)

Synonyms: FLJ41758, FLJ43475, FLJ44957

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN190856

Additional Information

Alternative name: SORCS1

Gene ID: 114815

Immunogen: C-QDPAGENKRSTDD

Format: Affinity-purified

Description: All names and symbols: SORCS1, sortilin-related VPS10 domain containing receptor 1 , RP11-446H13.1, FLJ41758, FLJ43475, FLJ44957 , OTTHUMP00000058855, SORCS receptor 1, VPS10 domain receptor SorCS, VPS10 domain receptor protein SORCS 1. Official Symbol: SORCS1. RefSeq number(s): NP_443150.3, NP_001013049.1

Specificity: This antibody is expected to recognise both reported isoforms (NP_443150.3, NP_001013049.1) Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an approx 25kDa band in human pancreas lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 130kDa according to NP_443150.3 and 133kDa according to NP_001013049.1. The 25kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Clee, Yandell, Schueler et al.: "Positional cloning of Sorcs1, a type 2 diabetes quantitative trait locus." in: Nature genetics, Vol. 38, Issue 6, pp. 688-93, 2006 (PubMed).