Corticotropin Releasing Hormone Receptor 1 (CRHR1) (AA 250-263) antibody

Antigen: Corticotropin Releasing Hormone Receptor 1 (CRHR1)

Synonyms: CRF1, CRHR, CRF-R, CRFR1, CRHR1f, CRH-R1h, CRFR2, crhr2, CRF2, crhr2-A, Crhr, MGC124237, MGC124240, CRHR1, crhr1-A

Epitope: AA 250-263

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine)

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN190893

Additional Information

Alternative name: CRHR1 / CRF-R (aa 250 - 263)

Gene ID: 1394, 12921 (mouse), 58959 (rat)

Immunogen: KLYYDNEKCWFGKR

Format: Affinity-purified

Description: All names and symbols: CRHR1, CRF-R, corticotropin releasing hormone receptor 1 , CRF1, CRFR1, CRH-R1h, CRHR, CRHR1f , corticotropin releasing hormone receptor variant 1h, seven transmembrane helix receptor. Official Symbol: CRHR1. RefSeq number(s): NP_004373.2

Specificity: Human, Mouse, Rat, Dog, Cow, Sheep 415 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments in human colon, duodenum and placenta lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? This product is successfully used in immunofluoresence and immunohistochemistry on mouse brain.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Ungless, Singh, Crowder et al.: "Corticotropin-releasing factor requires CRF binding protein to potentiate NMDA receptors via CRF receptor 2 in dopamine neurons." in: Neuron, Vol. 39, Issue 3, pp. 401-7, 2003 (PubMed).