Phosphodiesterase 4D3 (PDE4D3) antibody

Antigen: Phosphodiesterase 4D3 (PDE4D3)

Synonyms: DPDE3, STRK1, HSPDE4D, PDE4DN2, FLJ97311, DKFZp686M11213

Clonality: Polyclonal

Host: Goat

Reactivity: Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190895

Additional Information

Alternative name: PDE4D3

Gene ID: 5144, 24627 (mouse)

Immunogen: HVNNFPFRRHS-C

Format: Affinity-purified

Description: All names and symbols: PDE4D3, phosphodiesterase 4D3,. Official Symbol: PDE4D. RefSeq number(s): AAA97892.1

Specificity: This antibody is expected to recognize isoform 3 (AAA97892.1) only. Human, Rat 673 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 75kDa band observed in Rat Brain lysates (calculated MW of 76.5kDa according to human AAA97892.1). Recommended concentration: 0.01-0.03µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN190895 (0.01µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Houslay, Baillie, Maurice: "cAMP-Specific phosphodiesterase-4 enzymes in the cardiovascular system: a molecular toolbox for generating compartmentalized cAMP signaling." in: Circulation research, Vol. 100, Issue 7, pp. 950-66, 2007 (PubMed).