DiGeorge Syndrome Critical Region Gene 8 (DGCR8) antibody

Antigen: DiGeorge Syndrome Critical Region Gene 8 (DGCR8)

Synonyms: Gy1, DGCRK6, C22orf12, N41, D16Wis2, Vo59c07, D16H22S788E, D16H22S1742E, MGC78846, DGCR8, wu:fc23f08, wu:fc38f06, si:ch211-106a19.4, gy1, dgcrk6

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN190897

Additional Information

Alternative name: DGCR8

Gene ID: 54487, 94223 (mouse), 287954 (rat)

Immunogen: C-PDFVKQTSEEKPKD

Format: Affinity-purified

Description: All names and symbols: DGCR8, DiGeorge syndrome critical region gene 8, C22orf12, DGCRK6, Gy1,. Official Symbol: DGCR8. RefSeq number(s): NP_073557.3

Specificity: This antibody was designed using the mouse sequence according to NP_201581.2 but is also expected to recognize human Dgcr8 (GeneIG 54487, NP_073557.3, 86.0kDa). Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an unclear 90 kDa band a very strong 35kDa band in human liver lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the strong band we observe given the calculated size of 86.0kDa according to NP_073557.3. The 35 kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, DNA/RNA

Restrictions: For Research Use only

Publications

Publications: Yeom, Lee, Han et al.: "Characterization of DGCR8/Pasha, the essential cofactor for Drosha in primary miRNA processing." in: Nucleic acids research, Vol. 34, Issue 16, pp. 4622-9, 2006 (PubMed).