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Suppressor of Ty 16 Homolog (S. Cerevisiae) (SUPT16H) (Internal Region) antibody

Details for Product No. ABIN190913
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Antigen
Synonyms
CG1828, DRE4/dSPT16, Dmel\\CG1828, SPT16, Spt16, dSPT16, dSpt16, dspt16, l(3)62Bg, l(3)dre4, spt16, CDC68, SSF1, DDBDRAFT_0204912, DDBDRAFT_0231753, DDB_0204912, DDB_0231753, Cdc68, Fact140, Supt16h,  ... show more
CG1828, DRE4/dSPT16, Dmel\\CG1828, SPT16, Spt16, dSPT16, dSpt16, dspt16, l(3)62Bg, l(3)dre4, spt16, CDC68, SSF1, DDBDRAFT_0204912, DDBDRAFT_0231753, DDB_0204912, DDB_0231753, Cdc68, Fact140, Supt16h, DUF140, FACTP140, SPT16/CDC68 show less
Epitope
Internal Region
(7), (3), (3), (2), (1), (1), (1), (1)
Reactivity
Human
(50), (30), (14), (14), (14), (11), (2), (2)
Host
Goat
(37), (10), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(40), (16), (9), (8), (4), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
Options
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Catalog No. ABIN190913
374.27 $
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Immunogen Peptide with sequence C-DLTNKEGKKPEEK, from the internal region of the protein sequence according to NP_009123.1.
Cross-Reactivity Mouse (Murine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name FACT / SUPT16H
Background Synonyms: SUPT16H, FACT, suppressor of Ty 16 homolog (S. cerevisiae), HGNC:11465, CDC68, FACTP140, FLJ10857, FLJ14010, SPT16/CDC68, chromatin-specific transcription elongation factor large subunit, FLJ34357, facilitates chromatin remodeling 140 kDa subunit
Gene ID 11198
NCBI Accession NP_009123.1
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 150 kDa band observed in lysates of cell line HeLa (calculated MW of 120 kDa according to NP_009123.1). Recommended concentration: 0.1-0.3 µg/mL. An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunising peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Suppressor of Ty 16 Homolog (S. Cerevisiae) (SUPT16H) (Internal Region) antibody ABIN190913 (0.1µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Belotserkovskaya, Oh, Bondarenko et al.: "FACT facilitates transcription-dependent nucleosome alteration." in: Science (New York, N.Y.), Vol. 301, Issue 5636, pp. 1090-3, 2003 (PubMed).

Validation Images
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