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Suppressor of Ty 16 Homolog (S. Cerevisiae) (SUPT16H) (Internal Region) antibody

Details for Product No. ABIN190913, Supplier: Login to see
Antigen
  • CG1828
  • DRE4/dSPT16
  • Dmel\\CG1828
  • SPT16
  • Spt16
  • dSPT16
  • dSpt16
  • dspt16
  • l(3)62Bg
  • l(3)dre4
  • spt16
  • CDC68
  • SSF1
  • DDBDRAFT_0204912
  • DDBDRAFT_0231753
  • DDB_0204912
  • DDB_0231753
  • Cdc68
  • Fact140
  • Supt16h
  • DUF140
  • FACTP140
  • SPT16/CDC68
Epitope
Internal Region
14
6
6
5
4
3
3
1
1
1
1
Reactivity
Human
69
25
11
6
5
3
2
1
1
1
1
1
Host
Goat
38
16
16
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
63
32
10
10
8
2
2
1
Supplier
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Immunogen Peptide with sequence C-DLTNKEGKKPEEK, from the internal region of the protein sequence according to NP_009123.1.
Blocking Peptide Blocking peptide for this product available: ABIN368894
Cross-Reactivity Mouse (Murine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name FACT / SUPT16H (SUPT16H Antibody Abstract)
Background Synonyms: SUPT16H, FACT, suppressor of Ty 16 homolog (S. cerevisiae), HGNC:11465, CDC68, FACTP140, FLJ10857, FLJ14010, SPT16/CDC68, chromatin-specific transcription elongation factor large subunit, FLJ34357, facilitates chromatin remodeling 140 kDa subunit
Gene ID 11198
NCBI Accession NP_009123
Pathways
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 150 kDa band observed in lysates of cell line HeLa (calculated MW of 120 kDa according to NP_009123.1). Recommended concentration: 0.1-0.3 µg/mL. An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunising peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Suppressor of Ty 16 Homolog (S. Cerevisiae) (SUPT16H) (Internal Region) antibody (ABIN190913) ABIN190913 (0.1µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer). Pri...
Background publications Belotserkovskaya, Oh, Bondarenko et al.: "FACT facilitates transcription-dependent nucleosome alteration." in: Science (New York, N.Y.), Vol. 301, Issue 5636, pp. 1090-3, 2003 (PubMed).