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Piwi-Like 1 (Drosophila) (PIWIL1) antibody

Details for Product No. ABIN190916, Supplier: Log in to see
Antigen
  • HIWI
  • MIWI
  • PIWI
  • ziwi
Reactivity
Human
61
38
34
4
4
3
1
1
1
1
1
Host
Goat
53
12
4
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA
40
27
23
11
10
8
5
3
2
1
Supplier
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Immunogen Peptide with sequence C-SNRKDKYDAIKKY, from the internal region of the protein sequence according to NP_004755.2, NP_001177900.1.
Blocking Peptide Blocking peptide for this product available: ABIN369907
Specificity Human, Mouse, Rat, Dog
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Alternative Name PIWI / HIWI (PIWIL1 Antibody Abstract)
Background This antibody is expected to recognise both reported isoforms (NP_004755.2, NP_001177900.1). Synonyms: PIWIL1 antibody, HIWI antibody, PIWI antibody, piwi-like 1 (Drosophila) antibody, piwi (Drosophila)-like 1 antibody, homolog of Drosophila piwi antibody, MIWI antibody, piwi homolog, piwi-like 1 antibody.
Official Symbol: PIWIL1.
Gene ID 9271, 57749
NCBI Accession NP_004755
Application Notes Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Preliminary experiments showed a 100kDa band but also bands at approx 35kDa and 26-28kDa in Human Ovary, Placenta, Prostate and Testis lysates after 0.1µg/ml antibody staining.
Restrictions For Research Use only
Format Liquid
Concentration 100µg/200µl
Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Background publications Szakmary, Cox, Wang, Lin: "Regulatory relationship among piwi, pumilio, and bag-of-marbles in Drosophila germline stem cell self-renewal and differentiation." in: Current biology : CB, Vol. 15, Issue 2, pp. 171-8, 2005

Qiao, Zeeman, Deng, Looijenga, Lin: "Molecular characterization of hiwi, a human member of the piwi gene family whose overexpression is correlated to seminomas." in: Oncogene, Vol. 21, Issue 25, pp. 3988-99, 2002

Sharma, Nelson, Brandt, Wessman, Mahmud, Weller, Hoffman: "Human CD34(+) stem cells express the hiwi gene, a human homologue of the Drosophila gene piwi." in: Blood, Vol. 97, Issue 2, pp. 426-34, 2001