Glioma Tumor Suppressor Candidate Region Gene 2 (GLTSCR2) antibody

Antigen: Glioma Tumor Suppressor Candidate Region Gene 2 (GLTSCR2)

Synonyms: PICT1, PICT-1, R74911, AU041936, AW536441, 5330430H08Rik, 9430097C02Rik, GLTSCR2, pict1, pict-1, MGC133834, MGC115044

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN190917

Additional Information

Alternative name: PICT1 / GLTSCR2

Gene ID: 29997, 68077 (mouse), 292624 (rat)

Immunogen: CRRGPRNKKRGWRRL

Format: Affinity-purified

Description: All names and symbols: GLTSCR2, PICT1, glioma tumor suppressor candidate region gene 2, protein interacting with carboxyl terminus 1. Official Symbol: GLTSCR2. RefSeq number(s): NP_056525.1

Specificity: This antibody is expected to recognise a sequence corresponding to aa 38-51 of human PICT1 protein. Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 16-18kDa band in human heart, liver and breast cancer lysates after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 54.4kDa according to NP_056525.1. The 16-18kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Okahara, Ikawa, Kanaho et al.: "Regulation of PTEN phosphorylation and stability by a tumor suppressor candidate protein." in: The Journal of biological chemistry, Vol. 279, Issue 44, pp. 45300-3, 2004 (PubMed).