Interferon alpha (IFNA) antibody

Antigen: Interferon alpha (IFNA)

Clonality: Monoclonal (AB5)

Host: Mouse

Application: Enzyme Immunoassay (EIA), ELISA (Detection), Western Blotting (WB)

Catalog no.: ABIN191243

Additional Information

Alternative name: Interferon alpha (IFN alpha)

Immunogen: Hybridoma Clone s have been derived from hybridization of X63.Ag8.653 myeloma cells with spleen cells of Balb/c mice immunized with recombinant human interferon α-A.Myeloperoxidase free serum can be used for preparation of human myeloperoxidase standards and calibrators. Analysis: No detectable amounts of human myeloperoxidase.

Isotype: IgG1  (Matching secondary antibodies)

Clone: AB5

Specificity: MAb C10F5 recognizes common epitopes of α-A, -F, -D, -I1, -P human interferon subtypes. MAb C10F5 reacts with different antigenic epitope, than NK-2.YOK MAb (Celltech). Kd = 2.4 x 10 -10 M. IgG1 for MAbs C10F5 and AB5 IgG2a for MAbs AE3

Application Details

Application Notes: MAb C10F5 can be used for the detection of recombinant α-A, -F, - D, I1, -P interferon subtypes in two-site immunoassay and in affinity purification of recombinant interferons of α-A, -F, -D. MAbs AE3 and AB5 can be used in ELISA and in Western blotting. The recommended pair for sandwich immunoassay (coating – conjugate): AE3 – AB5 (detection limit 5 pg/ml).

Buffer: PBS, pH 7.4, 0.1 % sodium azide (NaN3) Material safety note: This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1 %) as preservative

Storage: + 4 °C

Restrictions: For Research Use only