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Antibodies
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anti-Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA) antibody
Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA) antibody
| Antigen |
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| Synonyms |
PAPA, DIPLA1, PAPP-A, PAPPA1, ASBABP2, IGFBP-4ase |
| Clonality |
Monoclonal (10A5) |
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Host
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Reactivity
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Application
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 Alternatives Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
Immunoprecipitation (IP) (26), Enzyme Immunoassay (EIA) (25), Flow Cytometry (FACS) (18), Immunofluorescence (IF) (18), Western Blotting (WB) (17), ELISA (Capture) (8), ELISA (Detection) (7), ELISA (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN191306 |
| Quantity |
1 mg |
| Price |
364.43 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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Pappalysin-1 / PAPPA
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Immunogen
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Hybridoma Clone s, producing MAbs, have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified human PAPP-A/proMBP (heterotetrameric PAPP-A, htPAPP-A) complex.
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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10A5
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Specificity
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Human PAPP-A antigen purified from placental blood (heterotetrameric complex, consisting of PAPP-A subunits and pro-MBP subunits). MAbs 11E4 and 5H9 are specific to proMBP subunit. IgG for MAb 11E4 IgG2a for MAbs 4G11, 10A5, 3C8, 10H9, 18A10 and 7A6 IgG2b for MAbs 5H9, 10E1 and 10E2
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Application Notes
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htPAPP-A and dPAPP-A (PAPP-A dimer) immunoassays and immunoprecipitation. Recommended pairs for the detection of dPAPP-A antigen in blood of AMI patients and patients with unstable angina (coating - conjugate): 4G11 – 3C8 4G11 – 10H9 10E1 – 7A6 Best pairs for the detection of htPAPP-A antigen in pregnancy serum (coating - conjugate): 4G11 – 3C8 4G11 – 10H9 10E2 – 10E1 10E2 – 5H9 5H9 – 10E2 10E1 – 7A6 PAPP-A immunodetection in Western blotting. MAb 18A10 recognizes PAPP-A subunit in reducing and non-reducing conditions after antigen transfer onto nitrocellulose membrane after SDS-gel electrophoresis. MAbs 4G11, 10A5, 18A10 and 10E1 recognize htPAPP-A, consisting of PAPP-A subunits and proMBP subunits, in non-reducing conditions.
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Buffer
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PBS, pH 7.4, 0.1 % sodium azide (NaN3) Material safety note: This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1 %) as preservative
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Storage
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+ 4 °C
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Restrictions
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For Research Use only
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Alternatives for antigen "Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA)", type "Antibodies"
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Hosts
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Mouse (29), Rabbit (20)
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Reactivities
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Human (49), Mouse (Murine) (20), Rat (Rattus) (19), Cow (Bovine) (3), Dog (Canine) (3), Horse (Equine) (3), Rabbit (2), Chicken (1), Pig (Porcine) (1), Sheep (Ovine) (1)
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Applications
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Immunoprecipitation (IP) (26), Enzyme Immunoassay (EIA) (25), Flow Cytometry (FACS) (18), Immunofluorescence (IF) (18), Western Blotting (WB) (17), ELISA (Capture) (8), ELISA (Detection) (7), ELISA (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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