Protein Kinase, CAMP-Dependent, Regulatory, Type II, alpha (PRKAR2A) antibody

Antigen: Protein Kinase, CAMP-Dependent, Regulatory, Type II, alpha (PRKAR2A)

Synonyms: PKR2, PRKAR2, MGC3606, PRKAR2A, prkar2a, MGC85886, zgc:85886, SLC25A20

Clonality: Monoclonal (RS-30)

Host: Mouse

Reactivity: Cow (Bovine), Human, Pig (Porcine)

Application: Enzyme Immunoassay (EIA), Functional Studies (Func), Immunofluorescence (IF)

Catalog no.: ABIN191325

Additional Information

Alternative name: PRKAR2A

Immunogen: Hybridoma Clone s have been derived from hybridization of P3X63Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with purified regulatory subunit of cAMP-dependent protein kinase type II from bovine brain.

Isotype: IgG2a  (Matching secondary antibodies)

Clone: RS-30

Specificity: Regulatory subunit of cAMP dependent protein kinase type II from bovine brain. Dissociation Constants of MAb Immune Complexes with pig and human RII in Comparison with Bovine RII. MAb KD, nM bovine RII KD pig RII KD bovine RII KD human RII KD bovine RII RS6 3.8 1.5 ± 0.87 64 ± 2.8 RS17 38.4 1.1 ± 0.71 0.9 ± 0.2 RS30 4.7 0.9 66 Influence of MAb on the Association of R-and C-subunits and cAMP binding. Antibody titre with RS MAb bovine pig human Inhibition of holoenzyme formation Influence on cAMP binding RS6 RS17 RS30 106 2.4x105 1.5x106106 7x105 1.5x1063x103 7x105 8x104Strong inhibition of holoenzyme formation No inhibition No inhibition Strong inhibition No influence Activation IgG1 for MAb RS6 IgG2a for MAbs RS30 and RS17

Application Details

Application Notes: Detection of regulatory subunit. MAbs work in ELISA and in indirect immunofluorescent staining.

Buffer: PBS, pH 7.4, 0.1 % sodium azide (NaN3) Material safety note: This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1 %) as preservative

Storage: + 4 °C

Restrictions: For Research Use only