COL17A1 antibody (Chain alpha 1)

Catalog No. ABIN191526

Product Details anti-COL17A1 Antibody

Target: COL17A1 (Collagen, Type XVII, alpha 1 (COL17A1))

Binding Specificity: AA 507-548, Chain alpha 1

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL17A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects Human type XVII Collagen / BP180 (aa 507-548).

Purification: Protein G Chromatography

Immunogen: Glutathione-S-transferase fusion protein containing the sequence of the immunodominant region of human type XVII collagen / BP180 (aa 507-548)

Application Details

Application Notes: ELISA: 1/5000. Western blot: 1/500. Immunocytochemistry on Frozen Sections: 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in aqua bidest to a concentration of 1 mg/mL.

Concentration: 2.0 mg/mL

Buffer: 50 mM Tris, pH 7.4

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized at 2-8 °C and reconstituted at -20 °C.

Target Details for COL17A1

Target: COL17A1 (Collagen, Type XVII, alpha 1 (COL17A1))

Abstract: COL17A1 Products

Synonyms: col17a1 antibody, fk89a06 antibody, si:ch211-223p8.6 antibody, wu:fk89a06 antibody, COL17A1 antibody, BA16H23.2 antibody, BP180 antibody, BPA-2 antibody, BPAG2 antibody, LAD-1 antibody, Bpag antibody, Bpag2 antibody, collagen type XVII alpha 1 chain antibody, collagen, type XVII, alpha 1b antibody, collagen, type XVII, alpha 1 antibody, Col17a1 antibody, col17a1b antibody, COL17A1 antibody

Background: Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in the gene coding for collagen XVII are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD 1, is generated by proteolytic processing of the full length form. Two transcript variants, one resulting from alternative splicing in the 3' UTR, have been identified for this gene.Synonyms: 180 kDa bullous pemphigoid antigen 2, BP180, BPAG2, Bullous pemphigoid antigen 2, COL17A1, LABD97, LAD-1

Gene ID: 1308

NCBI Accession: NP_000485

UniProt: Q9UMD9