Elastase, Neutrophil antibody
| Antigen |
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| Synonyms |
GE, NE, HLE, HNE, ELA2, PMN-E, Ela2, F430011M15Rik, ELANE, ela2, MGC89201, MGC157260 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Western Blotting (WB)
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| Catalog no. |
ABIN191647 |
| Quantity |
20 µl (Variants) |
| Price |
195.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: ELANE, ELA2, Neutrophil elastase, Elastase-2, PMN elastase, Bone marrow serine protease,Medullasin, Human leukocyte elastase, HLE
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Alternative name
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Neutrophil elastase
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Swiss-Prot
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P08246
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Immunogen
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Human purified PMN Elastase
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Cross-Reactivity
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Human
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Description
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Elastases form a subfamily of serine proteases that hydrolyse many proteins in addition toelastin. Neutrophil elastase (elastase 2) hydrolyses proteins within specialized neutrophillysosomes, called azurophil granules, as well as proteins of the extracellular matrixfollowing the protein's release from activated neutrophils. Elastase 2 may play a role indegenerative and inflammatory diseases by its proteolysis of collagen IV and elastin of theextracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. colias well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia.
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Specificity
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This antibody detects PMN Elastase. Species: Human. Others not tested.
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Application Notes
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ELISA (1/8.000). RIA (1/10.000). Western blot (1/500). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Purification
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Serum
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Storage
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Store lyophilized at 2-8°C and reconstituted at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
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Research Area
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Proteases, Enzymes
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Restrictions
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For Research Use only
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Alternatives for antigen "Elastase, Neutrophil", type "Antibodies"
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Hosts
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Mouse (23), Rabbit (7), Sheep (7), Goat (1)
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Reactivities
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Human (30)
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Applications
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Enzyme Immunoassay (EIA) (32), Radioimmunoassay (RIA) (14), Western Blotting (WB) (5), Functional Studies (Func) (3), To be determined by user (TBD) (3), ELISA (1), Flow Cytometry (FACS) (1), Immunodiffusion (ID) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1)
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Conjugates
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Biotin (2), HRP (1)
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