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Keratan Sulfate (KS) antibody
| Antigen |
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| Clonality |
Monoclonal (4B3-D10) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Western Blotting (WB)
Western Blotting (WB) (7), Flow Cytometry (FACS) (6), ELISA (5), Immunofluorescence (IF) (5), Immunohistochemistry (IHC) (4), Enzyme Immunoassay (EIA) (2), Radioimmunoassay (RIA) (2), Affinity Purification (AP) (1), Dot Blot (Dot) (1), Functional Studies (Func) (1), Immunocytochemistry (ICC) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Immunoprecipitation (IP) (1)
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| Catalog no. |
ABIN191938 |
| Quantity |
10 µg (Variants) |
| Price |
215.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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Keratan-Sulfate
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Immunogen
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Purified human aggrecan
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Cross-Reactivity
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Human
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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4B3-D10
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Description
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Aggrecan is the major proteoglycan of human articular car-tilage. The core protein issubstituted by a number of keratan sulfate and chondroitin sulfate glycosaminoglycanchains. Whereas chondroitin sulfate is widely distributed throughout the body, keratansulfate is primarily expressed in cartilage (joints, trachea, intervertebral discs) and cornea.
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Specificity
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This antibody is specific for keratan sulfate glycosaminoglycan chains. Preliminary results,based on western blot analysis of keratan sulfate from different sources, indicate that mAb4B3/10 is specific for keratan sulfate from articular cartilage and shows only minimal if anycross-reac-tivity with keratan sulfate from intervertebral disc. This is in contrast to theepitope recognized by the known mAb 5D4, that recognizes a widely distributed keratansulfate epitope. Therefore, in addition to appliations in immunochemistry andimmunohistochemistry, the mAb 4B3 / D10 is well suited for detecting keratan sulfatefragments released during human inflammatory or degenerative joint diseases intosynovial fluid and serum. Species: Human. Others not tested.
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Application Notes
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ELISA (less than 1 ug/ml). Westernblot (1 ug/ml). Immunohistochemistry (1 ug/ml). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Purification
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Purified
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Buffer
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50 mM TRIS pH 7,4Reconstitution: Restore in aqua bidest to 1 mg/ml.
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Storage
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Store lyophilized at 2 - 8°C and reconstituted at -20 °C. Avoid repeated freezing andthawing. Shelf life: One year from despatch.
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Research Area
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Extracellular Matrix
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Restrictions
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For Research Use only
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Alternatives for antigen "Keratan Sulfate (KS)", type "Antibodies"
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Hosts
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Mouse (7), Rabbit (5)
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Reactivities
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Human (12), Mouse (Murine) (7), Rat (Rattus) (6), Cow (Bovine) (2), Amphibian (1), Chicken (1), Dog (Canine) (1), Pig (Porcine) (1), Rabbit (1), Sheep (Ovine) (1)
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Applications
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Western Blotting (WB) (7), Flow Cytometry (FACS) (6), ELISA (5), Immunofluorescence (IF) (5), Immunohistochemistry (IHC) (4), Enzyme Immunoassay (EIA) (2), Radioimmunoassay (RIA) (2), Affinity Purification (AP) (1), Dot Blot (Dot) (1), Functional Studies (Func) (1), Immunocytochemistry (ICC) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Immunoprecipitation (IP) (1)
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Conjugates
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), PE,Cy5.5 (1)
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