Collagen, Type XVII, alpha 1 (COL17A1) (AA 1-452) antibody

Antigen: Collagen, Type XVII, alpha 1 (COL17A1)

Synonyms: BP180, BPAG2, LAD-1, FLJ60881, KIAA0204, BA16H23.2, Bpag, Bpag2, COL17A1, MGC155048, fk89a06, wu:fk89a06, si:ch211-223p8.6

Epitope: AA 1-452

Clonality: Monoclonal (V-5-8)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN192031

Additional Information

Characteristics: Synonyms: COL17A1, BP180, BPAG2, Bullous pemphigoid antigen 2, LAD-1, LABD97, 180 kDa bullouspemphigoid antigen 2

Alternative name: Collagen type XVII alpha 1 chain

Gene ID: 1308

Swiss-Prot: Q9UMD9

Immunogen: Human type XVII collagen intracellular domain (aa 1-490) GST fusion protein

Cross-Reactivity: Human

Isotype: IgG2a  (Matching secondary antibodies)

Clone: V-5-8

Description: Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is astructural component of hemidesmosomes, multiprotein complexes at the dermalepidermal basement membrane zone that mediate adhesion of keratinocytes to theunderlying membrane. Mutations in the gene coding for collagen XVII are associated withboth generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimericforms of type XVII collagen exist. The full length form is the transmembrane protein. Asoluble form, referred to as either ectodomain or LAD 1, is generated by proteolyticprocessing of the full length form. Two transcript variants, one resulting from alternativesplicing in the 3' UTR, have been identified for this gene.

Specificity: This antibody detects Human type XVII Collagen (234-398), Native Human type XVIICollagen. Species: Human. Others not tested.

Application Details

Application Notes: ELISA: 1/1000. Western blot: 1/100. Immunohistpochemitry on Frozen Sections: 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Buffer: 50 mM TRIS pH 7.4Reconstitution: Restore in aqua bidest to 1.0 mg/ml.

Storage: Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only

Images

Figure 1. V-5-8 mAb labels the dermal-epidermal junction by immunofluorescence microscopy. A 1:100 dilution of purified V-5-8 IgG was incubated 30 min at room temprature with frozen sections of human skin. After washing, bound IgG was visualized using FITC-conjugated antibodies specific for mouse (B) IgG and (C) IgG2a (all magnification, x400).