CD48 antibody (CD48 Molecule) (APC)

Details for Product anti-CD48 Antibody No. ABIN192135, Supplier: Log in to see
Antigen
  • AI449234
  • AW610730
  • BCM1
  • BLAST
  • BLAST-1
  • BLAST1
  • Bcm-1
  • MEM-102
  • SLAMF2
  • Sgp-60
  • hCD48
  • mCD48
  • CD48 antigen
  • Cd48 molecule
  • CD48 molecule
  • Cd48
  • CD48
Reactivity
Human, Non-Human Primate
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
This CD48 antibody is conjugated to APC
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42
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Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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Options
Supplier
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Immunogen Raji human Burkitt's lymphoma cell line
Clone MEM-102
Isotype IgG1
Specificity The antibody MEM-102 reacts with CD48 antigen (Blast-1), a 40-47 kDa GPI-anchored membrane protein (immunoglobulin supergene family) widely expressed on hematopoietic cells, it is negative on granulocytes, platelets and erythrocytes.
HLDA V, WS Code AS S014
Characteristics The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Alternative Name CD48 (CD48 Antibody Abstract)
Background CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily, which includes CD2, CD2F-10, CD58, CD84, CD150, CD229, CD244 and others. These molecules bind to the same or another members of their family, thus mediate homotypic or heterotypic adhesion. CD48 is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as a high affinity ligand for 2B4 and low affinity ligand for CD2. 2B4-CD48 interaction among NK cells and NK-T cells regulates cell proliferation. Signaling through CD48 results in eosinophil activation and CD48 expression is increased in several infectious diseases.
Gene ID 962
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-CD48 Molecule (CD48) antibody (APC) (ABIN192135) Surface staining of human peripheral blood cells with anti-CD48 (MEM-102) Alexa Fluor...
Product cited in: Schatzlmaier, Supper, Göschl, Zwirzitz, Eckerstorfer, Ellmeier, Huppa, Stockinger: "Rapid multiplex analysis of lipid raft components with single-cell resolution." in: Science signaling, Vol. 8, Issue 395, pp. rs11, 2015 (PubMed).

Carlsten, Baumann, Simonsson, Jaedersten, Forsblom, Hammarstedt, Bryceson, Ljunggren, Hellstroem-Lindberg, Malmberg: "Reduced DNAM-1 expression on bone marrow NK cells associated with impaired killing of CD34(+) blasts in myelodysplastic syndrome." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, 2010 (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Angelisová, Drbal, Horejsí, Cerný: "Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase." in: Blood, Vol. 93, Issue 4, pp. 1437-9, 1999 (PubMed).

Stulnig, Berger, Sigmund, Stockinger, Horejsí, Waldhäusl: "Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol." in: The Journal of biological chemistry, Vol. 272, Issue 31, pp. 19242-7, 1997 (PubMed).

Korínek, Stefanová, Angelisová, Hilgert, Horejsí: "The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1." in: Immunogenetics, Vol. 33, Issue 2, pp. 108-12, 1991 (PubMed).

Background publications Munitz, Bachelet, Eliashar, Khodoun, Finkelman, Rothenberg, Levi-Schaffer: "CD48 is an allergen and IL-3-induced activation molecule on eosinophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 1, pp. 77-83, 2006 (PubMed).

Lee, Forman, McNerney, Stepp, Kuppireddi, Guzior, Latchman, Sayegh, Yagita, Park, Oh, Wülfing, Schatzle, Mathew, Sharpe, Kumar: "Requirement of homotypic NK-cell interactions through 2B4(CD244)/CD48 in the generation of NK effector functions." in: Blood, Vol. 107, Issue 8, pp. 3181-8, 2006 (PubMed).

Mathew, Kumaresan, Lee, Huynh, Mathew: "Mutational analysis of the human 2B4 (CD244)/CD48 interaction: Lys68 and Glu70 in the V domain of 2B4 are critical for CD48 binding and functional activation of NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 2, pp. 1005-13, 2005 (PubMed).

Assarsson, Kambayashi, Persson, Chambers, Ljunggren: "2B4/CD48-mediated regulation of lymphocyte activation and function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 4, pp. 2045-9, 2005 (PubMed).

Bazil, Stefanová, Hilgert, Kristofová, Van?k, Bukovský, Horejsí: "Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102." in: Folia biologica, Vol. 35, Issue 5, pp. 289-97, 1990 (PubMed).

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