CD27 Molecule (CD27) antibody (Pacific Blue)

Antigen: CD27 Molecule (CD27)

Synonyms: T14, S152, Tp55, TNFRSF7, MGC20393, Tnfrsf7, CD27, MGC112688, MGC148404

Clonality: Monoclonal (LT27)

Host: Mouse

Reactivity: Human

Conjugate: Pacific Blue

Application: Flow Cytometry (FACS)

Certificates: ISO 9001:2008, ISO 13485:2003

Catalog no.: ABIN192226

Additional Information

Alternative name: CD27

Immunogen: Human peripheral blood lymphocytes

Isotype: IgG2a

Clone: LT27

Description: CD27 is a transmembrane 55 kDa protein of the nerve growth factor-receptor family, expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. This sCD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70.

Specificity: The antibody LT27 reacts with CD27 (T14), a 50-55 kDa type I transmembrane glycoprotein (member of the TNF-receptor superfamily) expressed on medullary thymocytes, peripheral T lymphocytes, some B lymphocytes and NK cells.HLDA V, WS Code T T-CD27.01

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 4 ml reagent / 100 ml of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Purification: The purified antibody is conjugated with Pacific Blueä under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only