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CD4 antibody (CD4 Molecule) (N-Term) (PE-DyLight 594)

Details for Product anti-CD4 Antibody No. ABIN192276, Supplier: Log in to see
Antigen
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Alternatives
anti-Human CD4 antibody for Flow Cytometry
Epitope
N-Term
66
61
21
17
15
15
15
13
12
10
9
8
8
8
8
6
6
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5
5
5
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4
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3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
1163
611
232
68
65
40
34
22
21
20
19
18
16
14
14
11
10
10
9
8
6
5
5
5
4
2
2
2
1
1
1
1
Host
Mouse
1315
570
271
10
4
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD4 antibody is conjugated to PE-DyLight 594
260
221
163
100
47
47
45
40
36
34
33
29
27
23
21
21
19
19
18
18
18
18
15
15
14
13
13
13
11
8
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5
5
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Western Blotting (WB)
1665
463
427
328
306
283
150
143
85
61
39
38
29
29
22
19
18
14
14
11
9
7
6
6
5
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Options
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Immunogen 2 N-terminal domains of human CD4 fused to human IgG1 Fc
Clone MEM-241
Isotype IgG1
Specificity The antibody MEM-241 recognizes CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (“,helper“, T-cells) and also on monocytes, tissue macrophages and granulocytes.

HCDM (former HLDA VIII) Meeting, May 2006, Québec, Canada, WS Code M241

Characteristics The purified antibody is conjugated with tandem dye PE-DyLight™, 594 (PE-DL594) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Maintenance of Protein Location
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 4 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (0.4 ml) is sufficient for 100 tests.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-CD4 antibody (CD4 Molecule) (N-Term) (PE-DyLight 594) (ABIN192276) Surface staining of human peripheral blood cells with anti-human CD4 (MEM-241) FITC.
Product cited in: Hovden, Karlsen, Jonsson, Aarstad, Appel: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." in: BMC immunology, Vol. 12, pp. 2, 2011 (PubMed).

Anderson, Sayers, Haniffa, Swan, Diboll, Wang, Isaacs, Hilkens: "Differential regulation of naïve and memory CD4+ T cells by alternatively activated dendritic cells." in: Journal of leukocyte biology, Vol. 84, Issue 1, pp. 124-33, 2008 (PubMed).

Karlsson, Cowley, Martinez, Shaw, Minger, James: "Homogeneous monocytes and macrophages from human embryonic stem cells following coculture-free differentiation in M-CSF and IL-3." in: Experimental hematology, Vol. 36, Issue 9, pp. 1167-75, 2008 (PubMed).

Manasa, Musabaike, Masimirembwa, Burke, Luthy, Mudzori: "Evaluation of the Partec flow cytometer against the BD FACSCalibur system for monitoring immune responses of human immunodeficiency virus-infected patients in Zimbabwe." in: Clinical and vaccine immunology : CVI, Vol. 14, Issue 3, pp. 293-8, 2007 (PubMed).

Zola, Swart, Banham, Barry, Beare, Bensussan, Boumsell, D Buckley, Bühring, Clark, Engel, Fox, Jin, Macardle, Malavasi, Mason, Stockinger, Yang: "CD molecules 2006--human cell differentiation molecules." in: Journal of immunological methods, Vol. 319, Issue 1-2, pp. 1-5, 2007 (PubMed).

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).

Background publications Clapham, McKnight: "Cell surface receptors, virus entry and tropism of primate lentiviruses." in: The Journal of general virology, Vol. 83, Issue Pt 8, pp. 1809-29, 2002 (PubMed).

Foti, Phelouzat, Holm, Rasmusson, Carpentier: "p56Lck anchors CD4 to distinct microdomains on microvilli." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 4, pp. 2008-13, 2002 (PubMed).

Millán, Cerny, Horejsi, Alonso: "CD4 segregates into specific detergent-resistant T-cell membrane microdomains." in: Tissue antigens, Vol. 53, Issue 1, pp. 33-40, 1999 (PubMed).