CD5 Molecule (CD5) antibody (Biotin)

Antigen: CD5 Molecule (CD5)

Synonyms: T1, LEU1, Ly-1, Ly-A, Ly-12, Lyt-1, MGC114334, CD5, MGC137790

Clonality: Monoclonal (CRIS1)

Host: Mouse

Reactivity: Human

Conjugate: Biotin

Application: Flow Cytometry (FACS), Western Blotting (WB)

Catalog no.: ABIN192377

Additional Information

Alternative name: CD5

Immunogen: stimulated human leukocytes

Isotype: IgG2a

Clone: CRIS1

Description: CD5 antigen (T1, 67 kDa) is a human cell surface T-lymphocyte single-chaintransmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most ofthymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is atype I membrane glycoprotein whose extracellular region contains three scavenger receptorcysteine-rich (SRCR) domains.The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsiccatalytic activity. CD5 modulates signaling through the antigen-specific receptor complex(TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosinephosphorylation of intracellular proteins and DAG production. Preliminary evidence showsprotein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dualreceptor, giving either stimulatory or inhibitory signals depending both on the cell type anddevelopment stage. In thymocytes and B1a cells seems to provide inhibitory signals, inperipheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is thephenotypic marker of a B cell subpopulation involved in the production of autoreactiveantibodies.Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferativedisorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in someautoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss ofCD5 expression in the expanded CD8+ human T cells.

Specificity: The antibody CRIS1 reacts with the cell surface glycoprotein CD5, a 67kDa single-chaintransmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes andB lymphocytes subset (B-1a lymphocytes).HLDA I, WS Code T 29HLDA III, WS Code T 530

Application Details

Application Notes: The reagent is designed for Flow Cytometry and. Suggested working concentration for Flow Cytometry is 2 µg/ml. Working concentrations should be determined by the investigator.

Concentration: 1 mg/ml

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Flow Cytometry analysis of human Peripheral Blood Lymphocytes (PBL) stained with CRIS1 antibody (dilution of purified antibody 1 ug/ml), Fig. 1a Histogram - gated on lymphocytes, overlay with isotypic control (mouse IgG2a), Fig. 1b Dot plot.

Publications

Publications: Alberola-Ila, Places, Cantrell et al.: "Intracellular events involved in CD5-induced human T cell activation and proliferation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 5, pp. 1287-93, 1992 (PubMed).

Arrizabalaga, Mirapeix, Darnell et al.: "Cellular immunity analysis using monoclonal antibodies in human glomerulonephritis." in: Nephron, Vol. 53, Issue 1, pp. 41-9, 1989 (PubMed).

Guarnue, Bravo, Calvo et al.: "Conformation of the hypervariable region L3 without the key proline residue." in: Protein science : a publication of the Protein Society, Vol. 5, Issue 1, pp. 167-9, 1996 (PubMed).