HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (AA 61-83) antibody

Details for Product No. ABIN192401
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Antigen
Synonyms MHC-G, B-F, B-F-S04, B-F-S05, B-F-S06, B-F-S07, B-FI, B-FIV, BF2, BFa2, BFw-03, BFw-05, BFz-01
Epitope
AA 61-83
(20), (10), (8), (6), (6), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(255), (4)
Host
Mouse
(191), (67), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(30), (28), (25), (8), (5), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunoprecipitation (IP), Western Blotting (WB)
(164), (108), (84), (63), (53), (47), (30), (27), (26), (24), (10), (8), (7), (1), (1), (1)
Pubmed 13 references available
Quantity 0.1 mg
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Catalog No. ABIN192401
377.52 $
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Immunogen amino acids 61-83 of HLA-G of human origin
Clone 4H84
Isotype IgG1
Specificity The antibody 4H84 recognizes HLA-G molecule (39 kDa). HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblast cells.This product is for research and in vitro experimental use only. It is not to be used for any other commercial purpose. Use of this product to produce products for sale or for therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the Univessity of California.
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name HLA-G
Research Area Major Histocompatibility Complex (MHC)
Application Notes Immunohistochemistry (paraffin sections) Recommended dilution: 10 µg/mL
ELISA:
Application note: The antibody 4H84 has been tested as the capture antibody in a sandwich ELISA for analysis of heavy chains of all HLA-G isoforms in combination with antibody MEM-G/1 .

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (AA 61-83) antibody Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by MEM-G/9 antibody and protein G. HLA-G was detected by 4H84 antibody and goat anti-mouse-HRP conjugate in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).
Product cited in: Guo, Chen, Ruan et al.: "HLA-G expression is irrelevant to prognosis in patients with acute myeloid leukemia." in: Leukemia research, 2011 (PubMed). (1:1000).

General Paul, Rouas-Freiss, Khalil-Daher et al.: "HLA-G expression in melanoma: a way for tumor cells to escape from immunosurveillance." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4510-5, 1998 (PubMed).

Urosevic, Kurrer, Kamarashev et al.: "Human leukocyte antigen G up-regulation in lung cancer associates with high-grade histology, human leukocyte antigen class I loss and interleukin-10 production." in: The American journal of pathology, Vol. 159, Issue 3, pp. 817-24, 2001 (PubMed).

Blaschitz, Hutter, Dohr: "HLA Class I protein expression in the human placenta." in: Early pregnancy (Online), Vol. 5, Issue 1, pp. 67-9, 2001 (PubMed).

Menier, Saez, Horejsi et al.: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules." in: Human immunology, Vol. 64, Issue 3, pp. 315-26, 2003 (PubMed).

Poláková, Krcová, Kuba et al.: "Analysis of HLA-G expression in malignant hematopoetic cells from leukemia patients." in: Leukemia research, Vol. 27, Issue 7, pp. 643-8, 2003 (PubMed).

Poláková, Kuba, Russ: "The 4H84 monoclonal antibody detecting beta2m free nonclassical HLA-G molecules also binds to free heavy chains of classical HLA class I antigens present on activated lymphocytes." in: Human immunology, Vol. 65, Issue 2, pp. 157-62, 2004 (PubMed).

Gonen-Gross, Achdout, Arnon et al.: "The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 8, pp. 4866-74, 2005 (PubMed).

Juch, Blaschitz, Daxböck et al.: "A novel sandwich ELISA for alpha1 domain based detection of soluble HLA-G heavy chains." in: Journal of immunological methods, Vol. 307, Issue 1-2, pp. 96-106, 2005 (PubMed).

Barrier, Kendall, Sharpe-Timms et al.: "Characterization of human leukocyte antigen-G (HLA-G) expression in endometrial adenocarcinoma." in: Gynecologic oncology, Vol. 103, Issue 1, pp. 25-30, 2006 (PubMed).

Gonen-Gross, Goldman-Wohl, Huppertz et al.: "Inhibitory NK receptor recognition of HLA-G: regulation by contact residues and by cell specific expression at the fetal-maternal interface." in: PLoS ONE, Vol. 5, Issue 1, pp. e8941, 2010 (PubMed).

Gan, Huang, Zhang et al.: "Tumor-specific upregulation of human leukocyte antigen-G expression in bladder transitional cell carcinoma." in: Human immunology, 2010 (PubMed).

Zheng, Wang, Zhang et al.: "Up-regulation of HLA-G expression in cervical premalignant and malignant lesions." in: Tissue antigens, Vol. 77, Issue 3, pp. 218-24, 2011 (PubMed).

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