HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody

Antigen: HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG)

Synonyms: MHC-G

Clonality: Monoclonal (4H84)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunocytochemistry (ICC)

Catalog no.: ABIN192401

Additional Information

Alternative name: HLA-G

Immunogen: amino acids 61-83 of HLA-G of human origin

Isotype: IgG1

Clone: 4H84

Specificity: The antibody 4H84 recognizes HLA-G molecule (39 kDa). HLA-G belongs to the MHC ClassI molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblastcells.

Application Details

Application Notes: Immunohistochemistry (paraffin sections) - ImmunocytochemistryELISA. Application note: The antibody 4H84 has been tested as the capture antibody in a sandwichELISA for analysis of heavy chains of all HLA-G isoforms in combination with antibodyMEM-G/1 (cat. no. 1B-291-C100).

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Major Histocompatibility Complex (MHC)

Restrictions: For Research Use only

Images

Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by MEM-G/9 antibody and protein G. HLA-G was detected by 4H84 antibody and goat anti-mouse-HRP conjugate in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).

Publications

Paul, Rouas-Freiss, Khalil-Daher et al.: "HLA-G expression in melanoma: a way for tumor cells to escape from immunosurveillance." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4510-5, 1998 (PubMed).

Urosevic, Kurrer, Kamarashev et al.: "Human leukocyte antigen G up-regulation in lung cancer associates with high-grade histology, human leukocyte antigen class I loss and interleukin-10 production." in: The American journal of pathology, Vol. 159, Issue 3, pp. 817-24, 2001 (PubMed).

Blaschitz, Hutter, Dohr: "HLA Class I protein expression in the human placenta." in: Early pregnancy (Online), Vol. 5, Issue 1, pp. 67-9, 2001 (PubMed).

Menier, Saez, Horejsi et al.: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules." in: Human immunology, Vol. 64, Issue 3, pp. 315-26, 2003 (PubMed).

Poláková, Krcová, Kuba et al.: "Analysis of HLA-G expression in malignant hematopoetic cells from leukemia patients." in: Leukemia research, Vol. 27, Issue 7, pp. 643-8, 2003 (PubMed).

Poláková, Kuba, Russ: "The 4H84 monoclonal antibody detecting beta2m free nonclassical HLA-G molecules also binds to free heavy chains of classical HLA class I antigens present on activated lymphocytes." in: Human immunology, Vol. 65, Issue 2, pp. 157-62, 2004 (PubMed).

Gonen-Gross, Achdout, Arnon et al.: "The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 8, pp. 4866-74, 2005 (PubMed).

Juch, Blaschitz, Daxböck et al.: "A novel sandwich ELISA for alpha1 domain based detection of soluble HLA-G heavy chains." in: Journal of immunological methods, Vol. 307, Issue 1-2, pp. 96-106, 2005 (PubMed).

Barrier, Kendall, Sharpe-Timms et al.: "Characterization of human leukocyte antigen-G (HLA-G) expression in endometrial adenocarcinoma." in: Gynecologic oncology, Vol. 103, Issue 1, pp. 25-30, 2006 (PubMed).

Gonen-Gross, Goldman-Wohl, Huppertz et al.: "Inhibitory NK receptor recognition of HLA-G: regulation by contact residues and by cell specific expression at the fetal-maternal interface." in: PLoS ONE, Vol. 5, Issue 1, pp. e8941, 2010 (PubMed).

Gan, Huang, Zhang et al.: "Tumor-specific upregulation of human leukocyte antigen-G expression in bladder transitional cell carcinoma." in: Human immunology, 2010 (PubMed).

Zheng, Wang, Zhang et al.: "Up-regulation of HLA-G expression in cervical premalignant and malignant lesions." in: Tissue antigens, Vol. 77, Issue 3, pp. 218-24, 2011 (PubMed).

Product: Guo, Chen, Ruan et al.: "HLA-G expression is irrelevant to prognosis in patients with acute myeloid leukemia." in: Leukemia research, 2011 (PubMed). (1:1000).