TCF4 / TF7L2 antibody

Antigen: TCF4 / TF7L2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: Immunoprecipitation (IP), Western Blotting (WB), Immunocytochemistry (ICC)

Catalog no.: ABIN192432

Additional Information

Immunogen: Bacterially expressed full-length human TCF4

Format: Purified

Description: TCF4 (T cell factor 4, also known as TF7L2) serves as transcriptional activator affecting e.g.cell cycle and terminal cell differentiation. In the absence of Wnt signals, TCF4 interacts withTLE/Groucho corepressor, supressing the transcription of TCF4 target genes. Wnt signalingleads to stabilisation and accumulation of beta-catenin, which binds TCF4 N-terminus,whereas TCF4 C-terminal HMG-box domains interact with DNA. Cell death-associated Daxxprotein or plakoglobin belong to negative regulators of TCF4-mediated transcription, on theother hand, there is a positive feedback loop of beta-catenin-TCF4 pathway comprising thep300 coactivator.

Specificity: Tha polyclonal antibody reacts with TCF4. TCF4 (transcription factor 7-like 2, 66 kDa)serves as a transcriptional activator affecting e.g. cell cycle and terminal cell differentiation.

Application Details

Application Notes: Immunoprecipitation - Positive control: DLD-1 human colon adenocarcinoma cell line. Application note: Reducing conditions. Immunocytochemistry - Recommended dilution: 1 µg/ml Staining technique: acetone / methanol fixation

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from rabbit serum by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only