RUFY1 antibody (AA 479-491)

Catalog No. ABIN1932338

Product Details anti-RUFY1 Antibody

Target: RUFY1 (RUN and FYVE Domain Containing 1 (RUFY1))

Binding Specificity: AA 479-491

Reactivity: Human, Rabbit, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This RUFY1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human RUFY1. This antibody is expected to recognize both reported isoforms (NP_079434.3, NP_001035541.1). Reported variants represent identical protein: NP_001035542.1, NP_001035541.1.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-QKNEAITSFEGKT from an internal region of human RUFY1 (NP_079434.3, NP_001035541.1). Percent identity by BLAST analysis: Human, Gibbon, Marmoset, Elephant, Rabbit (100%), Mouse, Hamster (92%), Rat, Panda, Pig, Opossum, Chicken, Platypus, Lizard (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (0.05 - 0.2 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 85kD band observed in lysates of cell line HeLa (calculated MW of 79.8kD according to NP_079434.3). Recommended concentration: 0.05-0.2 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for RUFY1

Target: RUFY1 (RUN and FYVE Domain Containing 1 (RUFY1))

Alternative Name: RUFY1 (RUFY1 Products)

Synonyms: RABIP4 antibody, ZFYVE12 antibody, 3000002E04Rik antibody, Rabip4 antibody, rabip4 antibody, zfyve12 antibody, RUN and FYVE domain containing 1 antibody, RUN and FYVE domain containing 1 L homeolog antibody, RUFY1 antibody, Rufy1 antibody, rufy1 antibody, rufy1.L antibody

Background: Name/Gene ID: RUFY1

Synonyms: RUFY1, FYVE-finger protein EIP1, RABIP4, La-binding protein 1, Rab4-interacting protein, ZFYVE12

Gene ID: 80230

NCBI Accession: NP_079434, NP_001035541