Hellethionin antibody

Antigen: Hellethionin

Clonality: Polyclonal

Host: Rabbit

Reactivity: Helleborus purpurascens

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN195437

Additional Information

Immunogen: mixture of extracted hellethionins A, B, C, D, E and G from Helleborus purpurascens conjugated to ovalbumin

Isotype: IgG  (Matching secondary antibodies)

Description: Hellethionins (Milbradt et el. 2003) belong to a family of plant thionins, a small-sized multiple-cysteine peptides that are involved in the plant defence against pathogens. The antimicrobial activity and cytotoxicity of thionins have led to the development of two potential applications of thionins: in agriculture transgenic plants containing thionin genes can enhance pathogen resistance, and targeting of thionins by tumor-specific antibodies is expected to support antitumor therapy.

Specificity: Reacts with Hellethionin A, B, C, D, E and G from H. purpurascens. The crossreaction with other plant thionins was not analysed.

Application Details

Application Notes: ELISA (1 : 256.000), Western blot (1 : 500 for ECL)

Buffer: contains 0.01% NaN3

Storage: short term +4°C; long term -20°C. Repeated freezing and thawing is not recommended.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

Western blot analysis extracted hellethionins B, C and D with anti-hellethionin. M– monomer, d – dimer forms of hellethionin.