C-Fos antibody
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (47), Rat (Rattus) (31), Mouse (Murine) (27), Pig (Porcine) (17), Dog (Canine) (5), Cow (Bovine) (3), Hamster (2), Cat (Feline) (1), Chicken (1), Horse (Equine) (1), Rabbit (1)
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Application
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Western Blotting (WB) (33), Flow Cytometry (FACS) (20), Immunofluorescence (IF) (19), Enzyme Immunoassay (EIA) (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), ELISA (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunohistochemistry (IHC) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (EM) (1)
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| Catalog no. |
ABIN195457 |
| Quantity |
200ug (1 mg/ml) (Variants) |
| Price |
455.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Application Notes
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Crude antiserum was purified by Affinity chromatography. Western blotting was performed against 40µg Hela lysate run on a NuPage 10% Bis-Tris gel at 200V for 30 mins in MOPS SDS running buffer. Transferred for 1.5 hours at 100V in tris-glycine buffer with 20% MeOH onto 45µm pore PVDF membrane. Blocked using 5% skimmed milk in TBS/tween. Incubated with 1º Ab at 1/100 dilution, O/N at 4ºC then 2º Ab 1/2000 horse anti-goat, 1 hour at RT.
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Concentration
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1 mg/ml
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Buffer
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Supplied in PBS containing 0.01% sodium azide and 1% trehalose.
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Storage
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The product should be stored at -20°C.
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Research Area
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Cancer
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Restrictions
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For Research Use only
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Alternatives for antigen "C-Fos", type "Antibodies"
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Hosts
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Rabbit (43), Mouse (8), Sheep (6)
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Reactivities
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Human (47), Rat (Rattus) (31), Mouse (Murine) (27), Pig (Porcine) (17), Dog (Canine) (5), Cow (Bovine) (3), Hamster (2), Cat (Feline) (1), Chicken (1), Horse (Equine) (1), Rabbit (1)
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Applications
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Western Blotting (WB) (33), Flow Cytometry (FACS) (20), Immunofluorescence (IF) (19), Enzyme Immunoassay (EIA) (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), ELISA (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunohistochemistry (IHC) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (EM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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N-Term (3), pT232 (1), pThr232 (1)
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Alternatives 
