E. coli (BL21) antibody

Antigen: E. coli (BL21)

Clonality: Polyclonal

Host: Rabbit

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN195458

Additional Information

Immunogen: E. coli expression strain BL21, whole protein lysate

Format: Liquid

Description: Determination of contaminating BL21 proteins in protein expression experiments, especially upon affinity purification of the target protein. The antiserum reacts strong and specific with BL21 proteins in Western Blotts and in ELISA applications.

Application Details

Purification: This antibody was produced by ProteinA/G dependent affinity chromatography of positive sera subsequently to immunization of NZW rabbits with E.coli expression strain BL21 whole cell lysate. The purity of antibody fractions was determined by Coomassie staining upon SDS-PAGE. Functional analysis of the purified antibody revealed strong and specific reactivity against BL21 proteins by Western Blotting and ELISA.

Buffer: TBS

Storage: At least 18 months when stored at –24°C. At least 4 weeks at 4°C when stored properly.

Research Area: Bacteria

Restrictions: For Research Use only

Images

Determination of residual BL21 proteins upon affinity purification by Western Blot. A protein sample of a affinity purified 40kD His-tag protein (A) expressed in the E.coli expression strain BL21 as well as a BL21 whole cell lysate (B) were resolved by SDS-PAGE and subsequently transferred to PVDF membrane. The target protein was determined by antiHIS-tag in combination with Anti-E.coli (BL21) antibody followed by Anti-Rabbit-HRP and colorimetric detection. The arrows indicate residual BL21 proteins in the purified fraction. Lane B shows the reactivity of Anti-E.coli (BL21) against a BL21 protein lysate. The numbers shown on the right represent marks of the protein standard used in the experiment.