HIS Tag antibody

Antigen: HIS Tag

Clonality: Polyclonal

Host: Rabbit

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Catalog no.: ABIN195462

Additional Information

Alternative name: HIS-tag

Immunogen: Cys – (Histidine)14

Format: Liquid

Description: Determination of recombinant proteins containing a 5 or 6 amino acid HIS-tag by Western Blotting, ELISA, Immunoprecipitation , Flow Cytometry, Immunohistochemistry

Application Details

Purification: This antibody was produced by antigen dependent affinity chromatography of positive sera subsequently to immunization of NZW rabbits with Cys–(Histidine)14 coupled to KLH. The purity of antibody fractions was determined by Coomassie staining upon SDS-PAGE. Functional analysis of the purified antibody revealed strong and specific reactivity against recombinant HIS-tag proteins by Western Blotting, Immunoprecipitation, ELISA, Flow Cytometry and Immunohistochemistry. Hereby it makes no difference if the HIS-tag of the target protein is fused to the N- or the C-terminus.

Buffer: PBS

Storage: At least 18 months when stored at –24°C. At least 4 weeks at 4°C when stored properly.

Research Area: Tags/Labels

Restrictions: For Research Use only

Images

Determination of a bacterially expressed recombinant 40kD protein with antiHIS-tag by Western Blot. Protein extracts of a negative (A) and a positive bacterial strain (B) were resolved by SDS-PAGE and subsequently transferred to PVDF membrane. The target protein was determined by antiHis-tag (1µg/ml) followed by Anti-Rabbit-HRP and ECL detection. The marks indicated on the left represent the prestained protein standard used in the experiment with individual bands of 23,1 kD, 31,3 kD, 53,6 kD and 100,4 kD respectively from bottom to the top.