HIS Tag antibody (HRP)
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Conjugate
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HRP (11), Biotin (10), FITC (8), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Alkaline Phosphatase (AP) (2), HiLyte Fluor 488 (2), HiLyte Fluor 555 (2), HiLyte Fluor 647 (2), PE,Cy5.5 (2), AMCA (1), Alexa Flour 488 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FAM (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1), ROX (1), Sepharose (1), TAMRA (1)
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Application
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 Alternatives Western Blotting (WB), ELISA, Flow Cytometry (FACS)
Western Blotting (WB) (109), ELISA (73), Immunoprecipitation (IP) (48), Immunofluorescence (IF) (45), Flow Cytometry (FACS) (30), Immunocytochemistry (ICC) (12), Immunohistochemistry (IHC) (10), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Dot Blot (Dot) (4), Transfected cell culture (TCC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunostaining (ISt) (1)
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| Catalog no. |
ABIN195464 |
| Quantity |
500ug (Variants) |
| Price |
1,369.33 $ Plus shipping costs $35.00 and if applicable $26.00 dry ice
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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HIS-tag
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Immunogen
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Cys – (Histidine)14
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Format
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Liquid
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Description
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Determination of recombinant proteins containing a 5 or 6 amino acid HIS-tag by Western Blotting, ELISA, Flow Cytometry.
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Purification
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This antibody was produced by antigen dependent affinity chromatography of positive sera subsequently to immunization of NZW rabbits with Cys–(Histidine)14 coupled to KLH. The purity of antibody fractions was determined by Coomassie staining upon SDS-PAGE. Subsequently the antibody was conjugated to HRP by periodate coupling. Functional analysis of the purified antibody revealed strong and specific reactivity against recombinant HIS-tag proteins by Western Blotting, ELISA and Flow Cytometry. Hereby it makes no difference if the HIS-tag of the target protein is fused to the N- or the C-terminus.
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Buffer
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PBS
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Storage
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At least 12 months when stored at –24°C. At least 4 weeks at 4°C when stored properly.
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Research Area
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Tags/Labels
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Restrictions
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For Research Use only
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Alternatives for antigen "HIS Tag", type "Antibodies"
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Hosts
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Mouse (78), Rabbit (53), Chicken (11), Goat (7), Sheep (1)
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Reactivities
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Fusion Protein (Tag) (14), Human (11), All Species (9), Mouse (Murine) (8), Rat (Rattus) (6), Tag (4), Cow (Bovine) (1), Dog (Canine) (1), Pig (Porcine) (1), Rabbit (1), Various Species (1)
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Applications
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Western Blotting (WB) (109), ELISA (73), Immunoprecipitation (IP) (48), Immunofluorescence (IF) (45), Flow Cytometry (FACS) (30), Immunocytochemistry (ICC) (12), Immunohistochemistry (IHC) (10), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Dot Blot (Dot) (4), Transfected cell culture (TCC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunostaining (ISt) (1)
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Conjugates
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HRP (11), Biotin (10), FITC (8), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Alkaline Phosphatase (AP) (2), HiLyte Fluor 488 (2), HiLyte Fluor 555 (2), HiLyte Fluor 647 (2), PE,Cy5.5 (2), AMCA (1), Alexa Flour 488 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FAM (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1), ROX (1), Sepharose (1), TAMRA (1)
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