Product details for  MDA5 (Human) antibody
Print Version Print Version       Download Datasheet Download Datasheet       Back to search results Back to search results       Add to basket Add to basket

MDA5 (Human) antibody

Overview

Image for MDA5 (Human) antibody
Antigen: MDA5
Antibody Type: Polyclonal
Application: Western Blotting (WB)
Reactivity: Human
Host: Rabbit
Quantity: 100ug
Price: 315,00 €   (Plus shipping costs and VAT)
Order number: ABIN195468
Availability: 10 days until shipment
Hints on ordering Hints on ordering
Add to basket

on this page

Additional Information
Application Details
Images
Related Products
External Information

Customer Service

Technical Inquiry
Send product details to colleague

Additional Information:  MDA5 (Human) antibody

back to top ^


Antigen MDA5
Immunogen Synthetic peptide of part of N terminal of human MDA5 (TIEDRNRIAAAENNGN)
Reactivity Human
Antibody Type Polyclonal
Format Affinity purified
Isotype IgG »Matching secondary antibodies
Description A full-length cDNA encoding MDA5 was isolated by screening a melanoma differentiation-subtracted library and a placenta cDNA library, also known as Ifih1(Interferon induced with helicase C domain protein 1). Yoneyama et al. have identified a DExD/H box-containing RNA helicase, retinoic acid inducible gene I (RIG-I), as an essential component of the sensor of intracellular dsRNA (1). Furthermore, two other DexD/H-box-containing RNA helicases, MDA5 and LGP2, were found in the mammalian database (2). MDA5 acts as a positive regulator in the virus-induced activation of type I IFN genes. On the other hand, LGP2 functions as a dominant-negative regulator of RIG-I/MDA5-mediated signaling. These molecules have critical roles for antiviral innate immune responses.
Host Rabbit

Application Details:  MDA5 (Human) antibody

back to top ^


Application Western Blotting (WB)
Application Notes Western blotting in concentration of 1 – 5?g/mL
Format Affinity purified
Purification Purified with antigen peptide
Buffer Lyophilized product from 1% BSA in PBS containing 0.05% NaN3
Storage Lyophilized product, 5 years at 2 – 8 ?. Solution, 2 years at –20 ?. 1.0 mL deionized water will be added to the product (the conc. comes up 100ug/ml)
Research Area Virology, Immunology
Restrictions For Research Use only

Images:  MDA5 (Human) antibody

back to top ^


lane 1: HeLa cell lysate without IFN beta stimulation. lane 2: HeLa cell lysate with IFN beta stimulation. When this antibody is used in Western Blotting, several bands appear although the antibody specifically detects the MDA5 protein. We recommend to perform positive and negative controls in parallel.

Image for MDA5 (Human) antibody (1)

External Information:  MDA5 (Human) antibody

back to top ^


Google Scholar Find more references for antigen MDA5 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen MDA5 (Bioinformatic Harvester (EMBL))